What is Cnidium Monnier Extracts?Pharmacology Actions and application of Cnidium Monnier and Osthol,C15H16O3,Osthola?

HPLC Determination of Osthole in Rat Plasma after Oral Administration of Fructus Cnidii Extract.

Common cnidium fruit Fructus Cnidii Osthol cnidium monnieri Cnidium Seeds Semen Cnidii Cnidium bhutanicum She ChuangShi SheSu Wild Carrot Seeds CNIDIUM MONNIERI FRUIT EXTRACT Osthole 484-12-8 photo picture image A simple and sensitive high-performance liquid chromatographic (HPLC) method is developed for the determination of osthole in rat plasma and applied to a pharmacokinetic study in rats after administration of Fructus Cnidii extract. After addition of fluocinonide as an internal standard, plasma samples are extracted with diethyl ether. HPLC analysis of the extracts is performed on a Hypersil ODS2 analytical column, using methanol-0.4% acetic acid (65:35, v/v) as the mobile phase. The UV detector is set at 322 nm. The standard curve is linear over the range 0.0520~5.20 ug/mL (r = 0.9979). The mean extraction recoveries of osthole at three concentrations were 81.0%, 91.2%, and 90.7%, respectively. The intra- and interday precisions have relative standard deviations from 1.9% to 4.9%. The limit of quantitation is 0.0520 ug/mL. The HPLC method developed can easily be applied to the determination and pharmacokinetic study of osthole in rat plasma after the animals are given the Fructus Cnidii extract. The plasma concentration of osthole from six rats showed a Cmax of 0.776 +- 0.069 ug/mL at Tmax of 1.0 +- 0.3 h.