Grapefruit and GSE.Function and Application of Grapefruit seed extract.

Contents

Research Update:Grapefruit Seed Extract.:

Grapefruit Seed Extract GSE INCI Name CITRUS GRANDIS SEED EXTRACT EINECS ELINCS No 289-904-6 CAS:90045-43-5 photo picture image

  Research Update:Grapefruit Seed Extract

 How Does it Work?
 Grapefruit Seed Extract (GSE):Discovery
 General use of Grapefruit Seed Extract:
 Preparation of Grapefruit Seed Extract:
 What microbes does grapefruit extract work against in your immune defense?
 Grapefruit Seed Extract May Help Ulcers Heal:An extract of grapefruit seeds may reduce acid production in the stomach.
 Grapefruit Seed Extract for Treatment of Thrush:
 Gastroprotective effects of flavonoids in plant extracts:
 Human Internal Uses Tips 1~50:
 Human External Uses Part 1:Tips:
 Human External Uses Part 2:Tips
 Benefits of Grapefruit Seed Extract:
 Baby Care Uses of Grapefruit Seed Extract:
 Natural Non Toxic Household Cleaner:Tips
 Alkalizing the blood:
 Candida Yeast Infections & Parasitic Infections:
 More Researches.


  How Does it Work?:

 It should come as no surprise that we know a lot more about what GSE is good for, than exactly how it works.
 Take aspirin. Since its development in 1899, billions of people have benefited from its ability to reduce inflammation, lessen pain, and lower fever. But exactly how it works is only recently being better understood.
 "Considering all the electron micrographs, we believe that the microbial uptake of GSE alters the cell membrane by inhibiting enzymatic activities?You can see the loss of the cytoplasmic membrane."
 What is not known is how GSE can affect the cell membranes of such a diverse group of microbes with virtually no toxicity toward animal life(The "Acute Oral Toxicity Study" performed by an independent laboratory concluded that you would have to take 4,000 times the normal adult dose to risk a 50% chance of poisoning). And to complicate matters further, since viruses do not have a cell membrane of their own, how is it that GSE can act as an antiviral remedy as it does?
 Unraveling the mysteries of GSE and its mechanisms would undoubtedly provide extremely valuable insight into the basic activity of microbes. We might hope that research funds will become available to answer the many questions raised about how it can be so potent and yet so safe.

  Grapefruit Seed Extract (GSE):Discovery

 Grapefruit Seed Extract (GSE) is prepared from the seeds, pulp, and white membrane from the fruits of the grapefruit. Due to the research instigated by a Yugoslavian born physicist named Dr. Jacob Harich (1919-1996), new light has been shed on the fruit of n. After World War II, Dr. Harich was enjoying a grapefruit for breakfast when he accidentally bit into one of the seeds. It was extremely bitter, which intrigued this young scientist. He was able to expand his research of the grapefruit seed after moving to Florida in 1963. Dr. Harich's claims prompted other institutions in Florida to research GSE. In 1990, GSE began receiving some attention from holistic health care practitioners who started recommending GSE to their patients for its powerful anti-microbial properties.
 To understand what makes GSE such an amazing product, it is helpful to know a little bit about seeds in general. Seeds contain the genetic codes to create another plant. In some cases, they are the only way that a plant has of reproducing. Seeds are obviously very important, and so many protect themselves from the various bugs and microbes that could destroy them with chemicals. In grapefruit seeds, these chemical protectors are called polyphenolic compounds. Many seeds have chemicals that provide protection; polyphenolic compounds in grapefruit seeds are special because they can easily be converted into a form that is safe for human use. The chemicals in other seeds are often considered toxic to humans and animals.
 GSE by itself is extremely acidic and is generally diluted with glycerine. For example, the GSE liquid we carry at the Co-op by NutriBiotic is 67% vegetable glycerine and 33% CITRICIDAL (the registered trademark form of GSE used in NutriBiotic products). "In hundreds of laboratory tests, GSE has demonstrated its ability to kill or inhibit the growth of a wide array of potentially harmful bacteria, fungi, viruses and protozoan parasites." (Sachs, 1997). A study by the Bio Research Laboratories of Redmond, Washington compared chlorine bleach to GSE and found that GSE was superior to chlorine bleach at killing organisms like E. coli. and Salmonella typhi. More and more studies like this are proving GSE more effective than common disinfectants like alcohol and bleach. Some farmers are beginning to use it as a way to extend the shelf life of their fruits and vegetables instead of chemical sprays.
 The uses for GSE are diverse and luckily it is more ecologically sound than some of the products that it could potentially replace. Veterinarians have used it to cure ear infections in cats and dogs, purify raw meat given to pets, and treat Giardia. It is becoming more popular as a disinfectant in hospital laundries and for household use as well. The potential for human use is also extensive and exciting. Its properties could potentially treat dandruff, sore throat, nausea, cold sores, and the list goes on and on. Its use as a water purifier is still being tested, but campers may want to add it to their packs to use as a first aid spray, food rinse, and biodegradable dish soap.
 As those of you who have tried to take this product internally can attest to, the taste is quite potent, so just make sure you follow the recommended dilutions. For those of you who just can't handle the taste, we also sell GSE in tablets. As always, be sure to consult your health care professional or veterinarian before using GSE for human or animal use.
 Grapefruit Extract.Grapefruit Seed Extract.CAS.NO.090045-43-5.GSE.Ethanol extract 10:1;Water extract 8:1;Citrus paradisi seed extract; Extract of grapefruit seed;Grapefruit peel extract;Citrus paradisi peel extract;Citrus decumana extract photo picture image img
Up.

  General use of Grapefruit Seed Extract:

 GSE is a broad spectrum bactericide, fungicide, antiviral, and antiparasitic compound. When used in vitro, GSE has been shown to be highly effective against a broad spectrum of bacteria, including Staphyloccus aureus, Streptococcus pyogenes, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Shigella dysenteriae, Legionella pneumoniae, Clostridium tetani, Diploccus pneumoniae, and many others. GSE also strongly inhibits many types of pathogenic fungi and yeast.

 Examples of external uses of GSE include:
 mouth and lips: mouthwash, mouth ulcers, thrush, bad breath, cracked lips, sunburned lips, and cold sores
 teeth and gums: plaque, tooth decay, toothaches, tooth extraction, gingivitis, and toothbrush cleaner
 nose and sinuses: sinusitis, runny nose (rhinitis), and nasal ulcer
 throat: sore throat, tonsillitis, coughs, hoarseness, and laryngitis
 ears: ear cleaning, earaches, and inflammation of the middle ear (otitis media) in conjunction with internal use
 face: acne and shaving
 scalp and hair: shampoo, dandruff, itching scalp, and head lice
 skin: small cuts, skin abrasions, scratches, minor burns, rashes, dermatitis, psoriasis, shingles, eczema, nettle rash, insect bites and stings, tick and leech bites, leg ulcers, warts, and skin fungi
 feet: athlete's foot, sweaty feet, calluses, corns, blisters, nail fungi, and cuticular infections
 vagina and genitals: vaginitis, yeast infections, vaginal parasites, feminine hygiene, and fungal and parasitic diseases in the male genital area
 External uses include but not limited to: Acne, Athletes Foot, Nail Fungus Infections. Skin Infections: Fungal / Bacterial / Parasitic / Viral / Ringworm, Cuts / Wounds. Warts, Poison Ivy/Poison Oak, Rashes, Jock Itch, Shaving Itch, Dandruff, Cold Sores / Herpes, Head Lice, Chickenpox, Cracked Lips, Toothbrush Cleaning and much more!
Up.

 Examples of internal uses include:

 acute and chronic inflammations in general
 colds and flu
 gastrointestinal infections
 vastritis and gastric and duodenal ulcers
 Candida albicans and other fungal diseases
 parasitic diseases
 allergies
 Dental Rinse: (For healthy gums and fresh breath):Stir 1-2 drops of extract into 2 oz or more of water. Vigorously swish the water around your mouth for about 10 seconds. 1-2 times daily .
 Throat Gargle:Stir 1 drop of extract into a small glass of water (3 oz or more) Gargle several times and use as often as needed.
 Ear Rinse:Thoroughly mix 1-3 drops of extract into 1 oz of vegetable glycerin or alcohol. Apply 1-2 drops of this solution into the ear 1-2 times daily. Use as often as needed
 Nasal Rinse:Mix 1 drop of extract with 6 oz of water. With head tilted back, fill one nostril at a time with an eyedropper full of mixed solution. Bring head up and down in a forward motion to force mixture into passages. Return head to normal position to allow the solution to drain. Do not inhale this mixture.
 Vaginal Rinse:Mix 1-2 drops of extract in 8 oz of water. Douche once daily for about a week.
 Internal uses include but not limited to : Digestive Upsets: Gastrointestinal Disorders, Diarrhea and Food Poisoning. Infections from: Parasites (single and multi-celled), Bacterial, Viral and Fungal. Candida Yeast Infections, Thrush and Chronic Fatigue. Oral Infections: Tooth Plaque, Gum Disorders, Breath Freshener and Mouthwash. Colds and Flu, Sore Throats, Strep Throat, Ear Inflammation / Pain, Gassiness, Sinusitis and much more!
Up.

  Preparation of Grapefruit Seed Extract:

 The extract is prepared by grinding grapefruit seeds and pulp into a fine powder. The powder is dissolved into purified water and distilled to remove fiber and pectin. The distilled slurry is spray dried at low temperatures forming a concentrated grapefruit bioflavonoid powder. This concentrated powder is dissolved in vegetable glycerin and heated. Food grade ammonium chloride and ascorbic acid are added. This mixture is heated under pressure where it undergoes catalytic conversion using natural catalysts, including hydrochloric acid and natural enzymes. The slurry is then cooled, filtered, and treated with ultraviolet light. Residual ammonium chloride in the final product is between 15 and 18%; residual ascorbic acid is between 25 and 35 mg/kg. There is no residue of hydrochloric acid in the final product. In the United States, standardized GSE contains 60% grapefruit extract materials and 40% vegetable glycerin. A powdered form of GSE is also available that contains 50% grapefruit extract materials, 30% silicon dioxide, and 20% vegetable glycerine.

  What microbes does grapefruit extract work against in your immune defense?:

 Bacteria:It can act against many gram negative bacteria,It can act against many gram positive bacteria,Campylobacter jejuni,Chlamydia trachomatis,Heliobacter pylori
 Fungus:It has worked against a number of fungi and yeasts. Aspergillus niger,Aspergillus fumigatus,Candida albicans,Epidermophyton floccosum,Keritinomyces ajelloi,Monilia albicans,Saccharomyces cerevisiae,Trichophyton mentagrophytes,Trichophyton rubrum,Trichophyton tonsurans
 Parasites:Entamoeba histolytica,Giardia lamblia
 Virus:Herpes simplex 1,Influenza A2
Up.

  Grapefruit Seed Extract May Help Ulcers Heal:An extract of grapefruit seeds may reduce acid production in the stomach.

 In their study some Polish scientists tested different doses of grapefruit seed extract on rats with stomach ulcers. Results showed a drop in gastric acid, which irritates and damages stomach tissue. Treatment with grapefruit seed extract also resulted in a shrinking of the ulcer size and an increase in blood flow to the ulcers, which may help them heal.
 Doses were scaled to the rats' weight. At a dose of 10 milligrams/kilogram, acid secretion was halved in rats with stomach ulcers. The ulcers also shrank progressively by the sixth and ninth days of treatment and had completely healed after 16 days, according to the study.
 The rats also showed a significant increase in blood flow to the ulcers, and they released more of an ulcer-healing chemical called gastrin, say the researchers, who included Thomas Brzozowski, MD, PhD, of Poland's Jagiellonian University Medical College.
 The extract could have something to do with Cox-1 and Cox-2 activity, say Brzozowski and colleagues.
 Cox-1 is an enzyme found in many parts of the body, including the stomach. It helps the stomach produce mucus, which protects it from tissue-damaging acids. Many common pain relievers, like aspirin, inhibit this enzyme, reducing mucus production in the stomach, which increases the risk of ulcers and severe irritation with bleeding.
 Cox-2 enzymes also produce chemicals linked to inflammation. The popular pain reliever Celebrex inhibits Cox-2.
 When the researchers paired the extract with drugs that inhibit the Cox enzymes, the extract's effects in healing ulcers were inhibited or completely wiped out.
 The findings may surprise many consumers as the citrus fruit is often associated with acidity and irritation of the gastrointestinal tract. But the seed extract actually possesses antibacterial and antioxidative properties that calm the gastric tract, said the researchers at the recent Digestive Disease Week conference in Chicago.
 The team from Jagiellonian University Medical College in Poland examined the effects of different doses of the extract (2-20 mg/kg) on induced gastric ulcers in rats.
 They measured levels of gastric secretion, one the major causes of gastric ulcers, and also tested ulcer healing by using a combination of acetic acid (an acid compound used to induce experimental ulcers) with or without a COX-1 inhibitor (indomethacin, 2 mg/kg) and a COX-2 inhibitor (rofecoxib, 10 mg/kg).
 Rats treated with the grapefruit extract at 10 mg/kg experienced a 50 per cent reduction in gastric acid secretion, and a progressive decrease in the area of gastric ulcers by days six and nine of treatment.
 The treatment also prompted a significant rise in blood flow at the ulcer sites, which could also help healing.
 But the effects were completely abolished by indomethacin, and significantly inhibited by rofecoxib.These drugs inhibit the enzymes COX-1 and COX-2, which play a key role in maintaining the health of the stomach lining.
 The researchers concluded that the extract accelerates ulcer healing via a mechanism involving COX-1 and COX-2 activity, combined with effects on gastric acid, gastric blood flow at the ulcer margin and release of gastrin, which promotes ulcer healing.
 “Because grapefruit is acidic in nature, people with ulcers might assume that they should not include the fruit in their diet,” said lead author Dr Thomas Brzozowski.
 “However, this research suggests the exact opposite. The antioxidant properties found in grapefruit and the ability of this fruit extract to limit oxidative stress in the ulcerative gastric mucosa have therapeutic properties that, when combined with additional therapies, can be especially beneficial for healing of gastric ulcers.”
 Ulcers can result when the stomach produces excess acid or there is insufficient mucus to protect the lining from damage. Infection with the Helicobacter pylori significantly raises the risk of ulcers, as does regular use of certain medicines like aspirin and other non-steroidal anti-inflammatory drugs (NSAIDs).
 Smoking, drinking and a fatty diet are also linked to ulcers.
 About 20 million Americans develop at least one ulcer during their lifetime and more than 40,000 Americans have surgery because of persistent symptoms or problems from ulcers every year.
Up.

  Grapefruit Seed Extract for Treatment of Thrush:

 Grapefruit seed extract can also be used for treating Candida as well. It can be used directly on the nipples and/or orally. If used directly on the nipples, it should be diluted (5 to 15 drops, occasionally up to 25 drops, in 30 ml or 1 ounce of water), applied on the nipples with a Q-tip or cotton ball, allowed to dry, and then covered, sparingly, with the all purpose nipple ointment. By mouth, grapefruit seed extract can be taken as a pill, 250 mg three times a day.
 Grapefruit seed extract should be used in conjunction with the APNO (All Purpose Nipple Ointment). Apply the diluted liquid grapefruit seed extract on the nipples, and then follow with the ointment (always after the feeding).
 Apply solution directly on the nipples. It does not need to be refrigerated. It may be covered and used until solution is finished.
 Mix very well five to 10 drops in 30 ml (1 ounce) of water (preferably, but not necessarily, distilled).
 Use cotton swab or Q-tip to apply on both nipples and areolas after the feeding.
 Let dry a few seconds, then apply "all purpose nipple ointment".
 If using Gentian Violet, do not use GSE on that particular feed but use after all other feeds.
 Should be used in conjunction with oral GSE, either tablets, capsules, or liquid extract (see above)
 Use until pain is gone and then wean down slowly over the period of at least a week.
 If pain is not significantly improving after two to three days, increase the dose by 5 drops per 30 ml (ounce) of water. Can continue increasing until 25 drops per 30 ml of water.
 If flaking, drying, or whiteness appears on the skin, substitute vitamin E oil or pure olive oil for APNO 1-3x/day.
 Laundry can be treated as well: add 15-20 drops in the rinse cycle of all wash loads
 If not using Gentian Violet, it may be helpful to treat baby with acidophilus by rolling a wet finger in acidophilus powder (break open a capsule), and let baby suck on the finger right before a feeding. Use 2x first day, 2x second day only. Mother may want to ingest Acidophilus as well, 3x/day for 1-2 weeks.

  Gastroprotective effects of flavonoids in plant extracts:

 The purpose of this paper is to overview the relations between plant-originated substances and their bioactivity measured in terms of antioxidant, cytoprotective and antiulcer activities. In addition, we assessed whether these compounds are capable of affecting the gastric mucosal lesions induced by absolute ethanol applied intragastrically (i.g.). The following plant-originated flavonoid substances were considered; Solon (Sophoradin extract), Amaranth seed extract, grapefruit-seed extract (GSE) and capsaicin (extract of chilly pepper). The area of gastric mucosa lesions and gastric blood flow were measured in rats with ethanol-induced lesions without (control) and with one of the tested substances without and with capsaicin denervation of afferent nerves or administration of L-nitro-arginine (L-NNA), an inhibitor of nitric oxide synthase (NOS). Male Wistar rats, weighing 180-220 g fasted for 24 h before the study where used 100% ethanol was applied i.g. to induce gastric lesions, whose area was determined by planimetry. Gastric blood flow was assessed using electrolytic regional blood flowmeter. All tested plant-originated substances afforded gastroprotection against ethanol-induced damage and this was accompanied by increase in gastric microcirculation, both changes being reversed by pretreatment with neurotoxic dose of capsaicin or by pretreatment with L-NNA. We conclude that plant-originated flavonoid substances are highly gastroprotective probably due to enhancement of the expression of constitutive NOS and release of NO and neuropeptides such as calcitonin gene related peptide (CGRP) released from sensory afferent nerves increasing gastric microcirculation.
Up.

  Human Internal Uses Tips 1~50:

 Allergies: Food sensitivities and hay fever. Mix (?) drops grapefruit seed extract in 1/2 cup water or juice 2-3 times daily with or without meals. Read more about allergies | Customer comments about allergies.
 Mouthwash and Dental Rinse: For healthy gums, fresh breath and to eliminate harmful germs and bacteria. Stir (?) drops grapefruit seed extract in 1 cup water. Vigorously swish a small amount of this dilution for 10 seconds or more 1-2 times daily. Also add (?) grapefruit seed extract to the reserve of "waterpik" type devices. Only for adults and children over 6 years old. Customer comments about dental hygiene.
 Bad Breath: Can be caused by bacteria inside the mouth, tooth decay or putrefaction in the lower digestive tract. Rinse the mouth and gargle with (?) drops grapefruit seed extract in 1 cup water several times daily. If the cause is in the digestive tract then take grapefruit seed extract internally at (?) drops mixed in 1/2 cup water or juice 2-3 times daily. Grapefruit seed extract is an extremely effective and long lasting breath freshener. Read more! about bad breath halitosis.
 Bladder Infection, Bacterial Cystitis and Incontinence: A frequent, urgent, burning and painful urination. As soon as you feel the symptoms take (?) drops of grapefruit seed extract and 1 tsp Baking soda in 1 cup water. Drink 3 times daily. Read more about bladder infections & incontinence..
 Bladder Infections, Interstitial Cystitis and Chronic Urethritis: Cloudy urine with foul odor. Pain goes away during urination, then immediately returns. Mix (?) drops grapefruit seed extract in 1 cup water. Drink 3 times daily, for one month. Read more about symptoms of chronic urethritis | bladder infections & interstitial cystitis.
 Candida Yeast and Fungal Infection: Read more about candida diet. 1st week: (?) drops grapefruit seed extract in 6 ounces of water, diluted unsweetened fruit or vegetable juice, once daily. 2nd week: (?) drops grapefruit seed extract twice daily. 3rd week (?) drops grapefruit seed extract three times daily. Note: Doses can be increased or decreased as necessary.
 Candidacies, Yeast Infections and Vaginal Rinse: Read more about candida diet. Mix (?) drops grapefruit seed extract in 1 cup water. Douche once daily for 1 week or as directed by your health care practitioner. Always dilute. For persistent cases use this recommendation together with the candida yeast and fungi infection recommendation (above) at the same time.
 Caries, Tooth Decay: Caries is a demineralization process of the tooth enamel and the hard substance of the tooth, caused by the metabolic process of plaque bacteria. Use 1 drop grapefruit seed extract on moist toothbrush; Brush thoroughly 3 times daily. After brushing rinse mouth using (?) drops grapefruit seed extract in 1 cup water. Or add (?) drops grapefruit seed extract to waterpik reservoir. For severe cases put (?) drops grapefruit seed extract in 1/4 cup water on a moist strip of cotton and place it on the gum for several minutes every day. Also See: Toothbrush cleaner below. Read more about bacteria and tooth decay.
 Chronic Fatigue Syndrome, Epstien Barr Virus, Hypoadrenalism and Hypotension: Mix (?) drops grapefruit seed extract in 1 cup water. Drink 3 times daily. Read more about chronic fatique syndrome, and epstien barr virus.
 Chronic Inflammation: Indicates a weakening of the immune system. Mix (?) drops grapefruit seed extract in 1 cup water or juice 2-3 times daily or more if necessary.
 Colds and Flu: Mix (?) drops grapefruit seed extract in 1 cup water or juice 3 times daily at the first sign of a cold or flu. As an alternative, Mix (?) drops grapefruit seed extract to a quart of fruit or vegetable juice and sip throughout the day. Read more about flu and customer comments about colds.
 Coughs, Hoarseness and Laryngitis: Mix (?) drops grapefruit seed extract in 1 cup of lukewarm water. Gargle several times daily. Read more about hoarseness, laryngitis & coughs.
 Cysts, Polyps, Benign Tumors, Lipomas and Wens: (To shrink) Mix (?) drops grapefruit seed extract in 1 Tbls. of olive oil, almond, sesame, avocado or vegetable oil. Rub on 2 or 3 times daily, especially if the growth is increasing in size, If this mix is irritating to the skin add a little more oil, if necessary. Read more about cysts & polyps.
 Diarrhea and Dysentery: Caused by contaminated food or water. Experts say a great many people in whom colitis or crohn's disease is diagnosed and the cause seems to be unknown are actually infected with shigella, amoebas, salmonella, fungi, giardia lamblia or other parasites. For which grapefruit seed extract is very effective. Mix (?) drops grapefruit seed extract in 5 oz. of water or juice 1-2 times daily. As a preventative measure mix (?) drops grapefruit seed extract per 5 oz. of water or juice and drink each day. Read more about diarrhea.
 Ear Rinse and Earaches: Thoroughly mix (?) drops grapefruit seed extract with 1 oz vegetable glycerin or alcohol. Apply (?) drops of mixture in affected ear as often as needed. Do not use full strength in ears.
 Flatulence and Gas: Mix (?) drops grapefruit seed extract in 1- cup water or juice 3 times daily, before or after meals. An appropriate probiotic is very helpful to replenish beneficial bacteria in the digestive tract. Digestive enzymes taken before meals are often helpful. If symptoms persist consult a Physician. Read more about the causes of flatulance.
 Gastritis, Gastric and Duodenal Ulcers: Helicobacter pylori is a bacteria that is now believed to be the cause of up to 90% of peptic and duodenal ulcers. When helicobacter is treated successfully, the sufferer usually finds permanent relief. Mix (?) drops grapefruit seed extract to 1 cup water. Drink a half an hour before a meal on an empty stomach 2 to 3 times daily until the symptoms have disappeared. read more about duodenal ulcers.
 If your stomach is sensitive start off with (?) grapefruit seed extract and increase as needed or take with or after meals. Note: Ulcers are very sensitive to acidic irritation, "extreme care" must be taken when using grapefruit seed extract. If irritation persist discontinue use. As ulcers can be a very serious condition. Consult a Physician.
 Gastrointestinal Disorder: If the cause seems to be unknown you may actually be infected with shigella, amoebas, salmonella, fungi, giardia lamblia or other parasites from contaminated food or water, etc. Mix (?) drops grapefruit seed extract in 1 cup water or juice 2 times daily. Read more about gastrointestinal disease.
 Gingivitis and Gum Disorders: Caused by plaque bacteria that can cause gums to bleed. Use (?) grapefruit seed extract on moist toothbrush; Brush thoroughly 3 times daily. After brushing rinse mouth using (?) drops grapefruit seed extract in 1 cup water. Or add (?) drops to waterpik reservoir. For severe cases use (?) drops grapefruit seed extract in 1/4 cup water on a moist strip of cotton and place it on the gum for several minutes every day. Also See: Toothbrush cleaner below. Customer comments about dental hygiene.
 Orally: For over all good health. Mix (?) drops grapefruit seed extract mixed in 1/2 cup water or juice. 2-3 times daily, with or without meals.
 Parasites: For internal use. Mix (?) drops grapefruit seed extract in 1 cup water or juice. 2-3 times daily. If you have a sensitive stomach take after meals until stomach adjusts to the extract and then you can take it independent of meals. Doses can be increased or decreased as necessary. Read more about parasites.
 Phlegm and Mucus Removal: Mix (?) drops grapefruit seed extract in 1-cup water and gargle deep in the throat using very little water mixture at a time. Repeat several times and slowly drink the rest.
 Plaque: A bacterial coating of the teeth that causes caries and periodontosis; a progressive gum disease. Put (?) grapefruit seed extract on moist toothbrush, brush extensively 3 times daily. Also put (?) drops grapefruit seed extract in 1 cup water rinse mouth thoroughly. Also: Read more about gum diseases & plaque. Customer comments about dental hygiene.
 Respiratory: People have successfully used grapefruit seed extract in a nebulizer to rid themselves of lung and bronchial infections. Symptoms disappeared in less than a day. Four of the five were children with chronic infections unaffected by antibiotics prescribed by their doctor. This method was first tried by a respiratory therapist. (1) drop grapefruit seed extract in one ounce saline water (for nebulizers). This solution could also be used in a vaporizer or boiling water. I cannot say if this would be effective against the sars virus but it may be worth a try? Customer comments about lung infections.
 Sinusitis and Nasal Rinse: Mix (?) drops grapefruit seed extract with 3/4 cup water. With the head tilted back fill each nostril with an eyedropper full of mix. Drop head forward and down (head is upside down) to force solution up into nasal passages. Return head to normal position and allow nasal passages to drain. Do not inhale during this process. Always dilute. Also: Read more about sinusitis. Customer comments about sinusitus.
 Sore Throat Gargle, Tonsillitis, Strep Throat, Swollen Glands (lymph Inflammation): Stir (?) drops grapefruit seed extract in 3 oz or more of water and gargle several times or as often as needed. Always Dilute. Increase dose if necessary. Read more about strep throat & tonsillitis. Customer comments about strep throat.
 Thrush: Is a fungal infection of the mouth region. Thrush appears as a whitish coating in the mouth and is caused by the yeast fungus; Candida Albicans. Thoroughly rinse the mouth about 3 times daily with (?) drops grapefruit seed extract in 1 cup water. For babies See: Baby care Also See: Candida yeast and fungal infections for more info. Read more about thrush.
 Toothaches: Mix (?) drops grapefruit seed extract in 1-cup water. Gargle several times a day. In addition: Soak a cotton pad in a mix of (?) drops grapefruit seed extract to 2-4 oz of water. This can be placed directly on the aching tooth. Read more about toothaches.
 Tooth Extraction: To prevent infection and relieve pain. Use (?) drops grapefruit seed extract in 1-cup water and gargle.
 Toothbrush Cleaner: Mix (?) drops grapefruit seed extract in 1-cup water. Submerge toothbrush for 10-15 minutes or leave in solution between uses. Rinse toothbrush before using. Change water and re-mix every few days. It is documented that up to 1 million bacteria can inhibit your toothbrush.
 Toxic Shock Syndrome and Virulent Staph Infection: After tampon use, douche with a solution of (?) drops grapefruit seed extract in 1-qt water. As a precautionary measure. Read more about toxic shock syndrome.
 Ulcerative Stomatitis: A contagious disease that causes the tongue and throat to become red, swollen, ulcerative and very sore. May affect the lips, palate and insides of the cheeks and produce swollen gums that bleed easily. Put (?) drops grapefruit seed extract in 1-cup lukewarm water. Mix well and rinse out the mouth. Gargle several times a day. This will promote healthy gums and fresh breath. Precautions: Sterilize toothbrush (see toothbrush cleaner above) to keep from spreading.
Up.

  Human External Uses Part 1:Tips

 Grapefruit seed extract used for many types of external skin conditions from known or unknown causes (fungal, parasites, bacteria, viral, etc). It is effective against fungal infections of the feet, nails and skin.
 It is also an excellent nontoxic spray for everyday burns, cuts, scrapes, insect bites and wounds. It stops infections dead. It is extremely beneficial in the healing process and greatly reduces than chance of reinfection.

 Grapefruit seed extract is a very good facial cleanser for general cleaning of oily skin and is very helpful in fighting acne infections. Simply apply 2-3 drops of GSE to moistened fingertips and gently massage the wetted facial area in circular motions for about a minute, rinsing thoroughly with cool water. Patients often report a tingling sensation of the skin following the treatment which is indicative of the cleansing action properties of the grapefruit seed extract.

 Acne can be caused by bacterial infections. A few drops of GSE added to your regular liquid cleanser makes a superior solution. GSE reduces bacterial growth and its astringent nature dries up glandular secretions reducing the opportunity for bacteria to breed. It also helps open pores keeping them clean and unclogged thus reducing blackhead and pimple formation!
 Physicians have observed that the herpes simplex virus becomes inactive just ten minutes after the application of grapefruit seed extract.
 Dandruff is most often caused by an inflammatory response to an overgrowth of scalp fungus which causes itching and scaling. When this is the case, good results may be obtained by adding a few drops of GSE to your regular shampoo, leave on for 3~5 minutes.
 It is used against head lice and mites (when no other remedy will work). Grapefruit seed extract is also nontoxic as opposed to over the counter ineffective remedies that are very toxic.
 Grapefruit seed extract is also used to effectively remove warts, corns and other nondescript skin abnormalities.
Up.

 Acne and Pimples: Thoroughly moisten face with warm or cool water. With hands still wet, apply a (few) drops grapefruit seed extract to the fingertips and gently message the facial area with circular motions. Rinse thoroughly and pat dry face. A slight tingling sensation indicates the deep cleaning properties of grapefruit seed extract. You may add (?) drops grapefruit seed extract to your regular liquid cleaner for a superior cleaning solution. Be very careful to not get grapefruit seed extract in your eyes. If you do rinse with warm water for 15 minutes and consult a Physician if Necessary.
 Athletes' Foot: Apply liberally after bathing. Add (?) drops grapefruit seed extract in 2-cups water in a spray bottle. Apply 2 times daily for 3 weeks or as required. Also add (?) drops grapefruit seed extract to a small load or (?) drops grapefruit seed extract to a large load of laundry to the last rinse when washing socks or stockings to avoid reinfection. As added protection put (?) drops grapefruit seed extract into wettened palm and work into a lather then rub entire foot. Let dry before putting shoes on.
 Sunshine is also very helpful. Also spray inside of shoes thoroughly. Socks can be soaked in the above solution. You can also moisten feet then put a (few) drops of undiluted grapefruit seed extract on feet and rub in. It is said that freezing your shoes kills athletes foot infection within the shoe?
 Blisters: Apply a (few) drops on the blisters for cleaning purposes. Read more about skin rash blisters.
 Body Wash: Add a (few) drops grapefruit seed extract to a sponge, cloth or special cleaning material such as is sold in health food stores etc. and wash entire body being very careful not to get GSE into the eyes. This will clean and remove scaly skin. If you get grapefruit seed extract into the eyes use plenty of warm or cold water to rinse them out good.
 Burns Superficial: Grapefruit seed extract is very effective for relieving the pain when applied to superficial burns. It can be applied undiluted providing that it does not come into direct contact with the eyes.
 Calluses: (Horny skin) A warm footbath lasting 5-10 minutes with (?) drops grapefruit seed extract per 1/2 gallon water is very helpful in removing the horny skin.
 Chicken Pox, Nettle Rash, Shingles and Hives: (Varicella-Zoster Virus). Mix (?) drops grapefruit seed extract with 1 tablespoon olive, sesame, almond, avocado or vegetable oil and apply to the skin or mix (?) drops grapefruit seed extract in 1-cup water and spray affected areas, as needed. Read more about chicken pox | shingles | nettle rash | hives.
 Cold Sores: (Herpes Simplex) Physicians have observed that the herpes simplex virus becomes inactive just ten minutes after the application of grapefruit seed extract. Mix a (few) drops grapefruit seed extract with vegetable glycerin (You can find it at health food stores or drug stores), olive, sesame, almond, avocado or vegetable oil. If it irritates add a little more oil. If it doesn't try a little stronger for quicker relief. Listen to your lips. Read more causes of fever blisters.
 Corns: Put (1) drop "undiluted" grapefruit seed extract on the corn once or twice a daily. Read more about corns.
 Cracked or Sunburned Lips: Mix (?) drops grapefruit seed extract in 1 Tbls. of almond, olive, sesame, avocado or vegetable oil and apply.
 Cuts, Wounds, Scrapes and Burns: Mix (?) teaspoons of grapefruit seed extract in 1-cup water or (?) teaspoon grapefruit seed extract in 1/2-cup water. Use distilled or boiled water. Add this mix to a spray bottle. Spray liberally over the affected area once every few minutes while performing other standard first-aid procedures. A deep wound would require a more dilute mixture to avoid irritation. Applying grapefruit seed extract directly on the burn can effectively eliminate pain from superficial burns. Pain will cease in about 15 minutes. For severe wound or burn see a physician.
 Dandruff and Itchy Scalp : Add (?) drops grapefruit seed extract to itching scalp each shampoo. Mix in hand or on head with shampoo. Message into scalp and leave on for at least 2 minutes. Rinse thoroughly. Note: May be used without shampoo (?) drops grapefruit seed extract per cup water rub into scalp and let dry. A spray bottle would be handy for this!
 Eczema and Dermatitis: A chronic itchy skin disease caused by food or chemical allergies. It usually appears on the inside of the elbow and knees and on the face or wrist. Try to eliminate offending foods or chemicals. Do not wash with soap. Mix (?) drops grapefruit seed extract with 2 oz. (1/4 cup) olive, sesame, avocado, almond or vegetable oil and rub into affected area twice daily.
 Facial Cleaner: Thoroughly moisten face with warm or cool water. With hands still wet, apply a few drops grapefruit seed extract to the fingertips and gently message the facial area with circular motions. Rinse thoroughly and pat dry face. A slight tingling sensation indicates the deep cleaning properties of GSE. Always dilute.
 First Aid: Abrasions and scratches. Mix (?) drops grapefruit seed extract in 1 Tbls water. Apply the mixture 2 times daily directly to the affected area of skin. Do not use full strength on skin. For large areas mix (?) drops grapefruit seed extract per cup distilled or (boiled and cooled water) and spray on affected areas.
 Head Lice: Add (?) GSE to (?) Shampoo. For thick or long hair double recipe). Thoroughly wet hair and pat dry so no water is dripping from hair. Mix well and spread evenly over the entire scalp and hair. Note: For children (when first applying mixture) have them hold a folded towel on forehead to keep excess extract out of eyes. After applying wipe off any excess mixture and cover the hair with plastic (to keep extract confined to the head). Let extract take effect for 30 minutes. Repeat after 3 days. Caution: If Grapefruit Seed Extract gets in to the eyes, wash them out immediately with plenty of water (warm if possible). Consult a Physician if Necessary.
 Impetigo: A skin infection usually affecting children. An itchy rash-like outbreak distinguished by a clear to yellow pus-like discharge that keeps the area wet. Either the Streptococcus or the Staphylococcus bacteria usually cause it. Mix (?) tsp. Grapefruit seed extract to 3 oz. water and spray several times daily.
 Excellent results have been reported by those who have added (?) tsp. Calendula Officianlis (Marigold) to the solution. Recurrent episodes of impetigo can be avoided by careful attention to hygiene. (?) tsp of grapefruit seed extract added to the wash cycle will help to decontaminate towels, linens etc. Allow them time to soak.
Up.

  Human External Uses Part 2:Tips

 Grapefruit seed extract is being used for many types of external skin conditions from known or unknown causes (fungal, parasites, bacteria, viral, etc). It is effective against fungal infections of the feet, nails and skin.

 It is also an excellent nontoxic spray for everyday burns, cuts, scrapes, insect bites and wounds. It stops infections dead. It is extremely beneficial in the healing process and greatly reduces than chance of reinfection.

 Grapefruit seed extract is a very good facial cleanser for general cleaning of oily skin and is very helpful in fighting acne infections. Simply apply 2-3 drops of GSE to moistened fingertips and gently massage the wetted facial area in circular motions for about a minute, rinsing thoroughly with cool water. Patients often report a tingling sensation of the skin following the treatment which is indicative of the cleansing action properties of the grapefruit seed extract.

 Acne can be caused by bacterial infections. A few drops of GSE added to your regular liquid cleanser makes a superior solution. GSE reduces bacterial growth and its astringent nature dries up glandular secretions reducing the opportunity for bacteria to breed. It also helps open pores keeping them clean and unclogged thus reducing blackhead and pimple formation!
 Physicians have observed that the herpes simplex virus becomes inactive just ten minutes after the application of grapefruit seed extract.

 Dandruff is most often caused by an inflammatory response to an overgrowth of scalp fungus which causes itching and scaling. When this is the case, good results may be obtained by adding a few drops of GSE to one’s regular shampoo, leave on for 3 – 5 minutes.

 It is used against head lice and mites (when no other remedy will work). Grapefruit seed extract is nontoxic as opposed to some over the counter ineffective remedies that are very toxic.
 Grapefruit seed extract is also used to effectively remove warts, corns and other nondescript skin abnormalities.
Up.

 Insect Bites and Stings: Apply grapefruit seed extract in an undiluted form. If the skin is sensitive, mix with a little bit of water or almond, olive, sesame, avocado or vegetable oil. Read more about insect bites.
 Itchy Scalp and Dandruff: Add (?) drops grapefruit seed extract to itching scalp each shampoo. Mix in hand or on head with shampoo. Message into scalp and leave on for at least 2 minutes. Rinse thoroughly. Note: May be used without shampoo (?) drops grapefruit seed extract per cup water rub into scalp and let dry. A spray bottle would be handy for this! Read more itchy scalp.
 Itchy Skin: Use (?) drops grapefruit seed extract per 2-cups water and spray itchy skin and rub in.
 Jock Itch: A few drops of grapefruit seed extract can be spread onto wet hands and rubbed into the penis for treatment purposes. Don't rinse it off afterwards. A mixture containing (?) drops of extract in an 1/4 cup of sesame oil is also suitable for this purpose. Let it take effect for several minutes. Grapefruit seed extract should never be poured onto the penis in its undiluted form since this could lead to intensive irritation (ouch!). Measures should be continued for about 2 weeks. In addition, add (?) drops of grapefruit seed extract to the last rinse when washing you underwear for the next several weeks in order to prevent reinfection.
 Nail Cleaner: To clean whiten toes and fingernails. Use a few drops grapefruit seed extract and scrub nails with a small brush. Let stand for a few minutes before wiping off excess grapefruit seed extract, unless you plan on using nail polish afterwards?
 Nail Fungi: Deformed discolored soft or brittle nails. Mix (?) drops grapefruit seed extract in 1 Tbls of water, grain alcohol or vodka. Apply the solution on the surface of the nail, along cuticle or soak nails 2 times daily. Nail fungus is particularly resistant; several months of regular use may be necessary. For resistant cases add more drops to the above mixture or it may be used full strength if necessary. Eating antifungal foods such as; onions and garlic is helpful.
 Nettle Rash, Chicken Pox, Shingles and Hives: (Varicella-Zoster Virus). Mix (?) drops grapefruit seed extract with 1 tablespoon olive, sesame, almond, avocado or vegetable oil and apply to the skin or mix (?) drops grapefruit seed extract in 1-cup water and spray affected areas, as needed.
 Poison Ivy and Poison Oak: Mix (?) tsp grapefruit seed extract to 5 oz. (10 Tbls) water or (?) grapefruit seed extract to 2 1/2 oz. (5Tbls) water in a spray bottle and apply to a small area of the skin. If a burning sensation results, add a little water. Spray liberally over affected areas. Repeat every 2 hours as needed.
 Psoriasis: An itchy skin disorder characterized by red patches covered by silvery white scales. Mix (?) drops grapefruit seed extract with 1/4 cup olive, sesame, avocado, almond or vegetable oil and rub into affected area twice daily. Read more about psoriasis.
 Rashes: Cracking, dry flaky scaly skin. Mix (?) drops grapefruit seed extract with 1 tablespoon olive, sesame, almond, avocado or vegetable oil and apply to skin 2-3 times daily.
 Ringworm: This is a fungus not a worm. A ring like shape, anywhere on the skin. Mix (?) drops of grapefruit seed extract to 2 1/2 oz. (5 Tbls) water in a spray bottle. Spray affected area. If burning sensation is felt add a little more water. Olive, sesame, almond, avocado or vegetable oil can be used in place of water and rubbed into affected area. Read more about skin disorder fungus and Ringworm
 Shaving Itch: Shaving itch; a micro-infection usually caused by fungus. Thoroughly clean razor and blades before and after using. Bacterial infections leading to impetigo may occur Read more about impetigo. Add (?) drops grapefruit seed extract to shaving cream or use facial cleanser dose above for an after shave.
 Skin Fungi: Moist weepy red patches on the body. Rub undiluted grapefruit seed extract onto the affected spot or mix (?) tsp grapefruit seed extract to 2 1/2 oz. (5Tbls) water, vegetable glycerin or olive, sesame, avocado, almond or vegetable oil.
 Use 2 times daily on a regular basis. This should be continued for some time after symptoms have disappeared since the skin fungi often has not died completely and could grow again. Treat all areas simultaneously to keep from spreading. Expose the skin to a great deal of fresh air and sunshine. Read more about skin disorder fungus and Ringworm
 Skin Moisturizer: Mix (?) drops grapefruit seed extract to 16oz (2 cups water) and spray the skin. It is refreshing and cleansing. Caution: Do not get the spray into the eyes.
 Ticks and Leeches: Put grapefruit seed extract directly onto the tick or leech. Get as close as you can to your skin to remove. Do not squeeze the body. Then remove and put another drop on the bite. Note: Deer ticks can also transmit the even more serious pathogen; Eherlichia that causes HGE that can be fatal. Thoroughly check for tiny deer ticks if you live on an infested area. As an added precaution, mix (?) drops grapefruit seed extract in 8 oz. water. Drink 3 times daily for 1 or 2 weeks as precaution.
 Grapefruit seed extract has also been used for Lyme disease caused by ticks. It appears that grapefruit seed extract can disorganize the organism's cell wall and allow leakage of cellular contents, ultimately resulting in the death of the cell. We humans have no cell wall and thus our cells are not affected. Read more about lyme disease.
 Underarm Deodorant: A nontoxic alternative. Mix (?) drops grapefruit seed extract or more if necessary in 2-cups water in a spray bottle. Shake well and spray under the arms (it works very well).
 Varicose Veins: Soak a clean compress or wound pad with a mixture of (?) grapefruit seed extract to 2-4 oz. of boiled and cooled water, place in the affected region. Renew repeatedly.
 Common Warts: Apply a few drops of full concentrate grapefruit seed extract directly onto the wart. Cover with a band-aid. Apply 2 times daily, for 3 weeks or as required. Caution: Warts are highly contagious viruses that can spread if picked, trimmed, bitten or touched. Read more about common warts.
 Warts need to be kept moist with grapefruit seed extract. Sometimes it is necessary to trim excess skin off of the wart, be very careful to sterilize clippers, etc. before and after use and disposes of clippings properly. Warts are tough customers that need constant attention to get rid of. Grapefruit seed extract is very capable of dealing with warts if used properly.
Up.

  Benefits of Grapefruit Seed Extract:

  1. Broad Spectrum:Grapefruit seed extracts main advantage is it's extraordinary ability to perform both (internally and externally) against a wide variety of known or unknown infections caused by viruses, bacteria, funguses and parasites.

  2. Grapefruit seed extract is alkalizing:Grapefruit seed extract helps alkalize the body. It is considered one of the most alkaline forming foods and of all fruits it is the most alkalizing. Alkalizing the body (raising pH) is one of the single most important health regeneration benefits available.
 Disease cannot live in an alkaline environment within the body. It is claimed that cancer dies in a pH of 8.0. Countless other conditions are caused by an acid environment within the body. These same conditions are effectively treated by restoring the proper pH.
 Grapefruit seed extracts pH is a low 2.0. Don't let this fool you as acid foods have alkaline effects on the body. The problem comes when you eat food that is acid forming such as meat, sugar and most grains. This food creates and acid environment within the body.
 Most degenerative diseases are associated with your body pH. Your body pH is one of the most important aspects to consider when trying to overcome a serious illness and to maintain good health. The list of diseases caused from improper pH is a mile long. Cancer and most other diseases cannot exist in an alkaline environment. The pH of the non-deficient and healthy person is in the 7.5.

  3. Beneficial Bacteria:Grapefruit seed extract stimulates the immune system. (According to Doctors and Veterinarians) grapefruit seed extract (at normal doses) is gentle yet preserves the integrity of your intestinal bacteria. Without these beneficial bacteria life could not be sustained.

  4. Drug Resistant Bacteria:To date there is no evidence that any type of pathogenic microorganism has ever built up a resistance to grapefruit seed extracts active ingredient. Due to the mode of its activity it is believed that this resistance is impossible as grapefruit seed extract disrupts the organisms cytoplasmic membrane.

  5. It can be taken for long periods of time without toxic side effects:There are reports that people have taken grapefruit seed extract every day for several years (as a preventative) without side effects of any kind.

  6. GSE is hypoallergenic (non-allergenic):Dr. Allen Sachs points out that about 3 to 5 per cent of all people are allergic to citrus fruit and could therefore also display a sensitive reaction to grapefruit seed extract. These people should start with a low dosage and perform a patch test to be sure.

  7. It can be used for humans and animals including pets (fish, reptiles, birds etc):All living creatures great and small have benefited from the use of grapefruit extract.

  8. Grapefruit seed extract is a multipurpose product used for countless applications:
 Pet Health - Internal and external conditions and infections, skin conditions, mange, fleas, etc.
 Household Uses - Vegetable wash, counter tops, cutting boards, laundry cleaner, etc.
 Travel Uses - Grapefruit extract is used against water and food borne illnesses, diarrhea, dysentery, etc.
 Gardening Uses - Tool cleaner to remove fungus, mold. As a bug spray, plant spray and more!

  9. Wound Healing:Grapefruit seed extract is extremely good for fast healing of scar tissue associated with many types of wounds (even big ugly scars).

  10. It is Very Economical to Use:Grapefruit seed extract is very effective at low concentrations therefore a little goes a long way as most applications only require a few drops per dose. Our 1.5 oz bottle contains 900 drops of potent Triple Strength grapefruit seed extract. Triple Strength averages around 3-5 drops per dose.
Up.

  Baby Care Uses of Grapefruit Seed Extract:

 It is important to keep babies clean and healthy.
 Grapefruit seed extract is being used to clean baby bottles, nipples, pacifiers, toys, diapers, clothing and bedding.
 It can be used to spray or wipe off cribs, plastic mattresses and for many other general cleaning purposes.
 In countless lab test grapefruit seed extract has been proven to be at least 10 times more effective than Colloidal Silver, Iodine and Clorox Bleach. Just the smell of these toxic cleaners are harmful to your health.
 Here's a list of common household products that contain known carcinogens or neurotoxins composed by David Steinman, author of The Safe Shopper's Bible.
 Grapefruit seed extract is nontoxic and can be used safely by most people. Even infants and the elderly, as well as those with compromised health.
 A growing number hospitals are now using grapefruit seed extract as a safe alternative to the harsh and damaging chemical based cleaners of the past. Grapefruit seed extract is being used in the wash to insure that linens are fungi and bacteria free. It also eliminates molds and odors associated with rancid laundry.
 Jerry Skidmore, C.L.M Manager of laundry operations for the Florida Hospital, wrote, "I have had 30 years experience in the laundry industry and it is only since using (Grapefruit Seed Extract) that I have had the peace of mind and assurance that the patients in our hospital and the other hospitals we serve have complete protection from fungal and bacteria infections that can be associated with linens. It is especially gratifying to know that even after many hours of exposure to the various bacteria that are always present in hospitals, our linens has been tested and found free of all harmful or pathogenic organisms."
 Hospitals also add grapefruit seed extract to their carpet shampooing machines. It is reported that just 10-15 drops of grapefruit seed extract per reservoir gallon is effective against many pathogenic organisms.
 Warning: Always dilute grapefruit seed extract with water or carrier oil. Never apply full strength to the baby's skin and never get it in baby's eyes. If it gets into the eyes, wash immediately with plenty of water (warm if possible) and consult a Physician.
Up.
 Cradle Cap: Is an infant's form of dandruff. To eliminate mix (?) drops GSE in a little oil and rub into the head until condition clears up. Read more about cradle cap.
 Diaper Rash: Mix (?) drops of grapefruit seed extract in 2-4 oz. almond, olive, sesame, vegetable or avocado oil and gently rub it into the skin. Read more about diaper rash.
 Bottle / Nipples / Pacifier Wash: To mix use (?) drops grapefruit seed extract in 2-cups of water. Leave them in the solution for 20 minutes (rinse well afterwards).
 Thrush: This is a fungus that can be seen as a whitish coating in the mouth. Mix no more than (?) drops of grapefruit seed extract to 1-cup water. Then rub with your finger around in the mouth, as well as possible with the solution. Children over 6 years old can also gargle or swish with the mixture. Caution: Be extra careful to mix grapefruit seed extract very thoroughly in the water solution before using as babies mouths are very sensitive to grapefruit seed extract.
 The fungus likes to settle on pacifiers and nipples of baby bottles, leading to constant re-infection. To clean baby nipples and pacifiers from thrush put them into a solution of (?) drops grapefruit seed extract to 1 qt. water. Soak for 20 minutes and rinse very well before using. Read more about thrush.
 Toy Wash: Objects that babies frequently put in their mouths can be effectively cleaned with a solution of (?) drops grapefruit seed extract in 1 qt. of water. Leave them in the solution form 10 minutes an then let them dry in the air.
 Diaper / Clothing Wash: Add to final rinse cycle, (?) or more drops of grapefruit seed extract to a small load and (?) drops of grapefruit seed extract to a large load. As hot water temperatures do not always remove, viruses, bacteria and funguses etc.
Up.

  Natural Non Toxic Household Cleaner:Tips

 Grapefruit seed extract is a natural cleaning formula used to protect against Staph, Strep, Aspergillus, Salmonella, and many other common bacteria viruses and parasites found in the household.

 It is simple to use, put a few drops in a spray bottle and use it as a spray on wipe off cleaning solution, there's no need to rinse it off. It is also very economical to use in comparison to other cleaners.

 Most all household cleaners contain hazardous, caustic and obnoxious smelling chemicals that can be absorbed into your body either by touch or smell. They burn your skin, eyes, mucus membranes and can also be fatal if swallowed or cause other health problems such as cancer if used over long periods of time. Just the smell of these toxic cleaners are harmful to your health. Grapefruit seed extract is nontoxic, does not smell and it leaves no toxic residue.

 Here's a list of common household products that contain known carcinogens or neurotoxins composed by David Steinman, author of The Safe Shopper's Bible.

 In pathology, a carcinogen is any substance or agent that produces cancer. A neurotoxin is a toxic agent or substance that inhibits, damages or destroys the tissues of the nervous system, especially neurons, the conducting cells of your body's central nervous system. Neurotoxic effects can include behavior changes, seizures, as well as wide range of effects, including death. Symptoms include tremors, incoordination, elevated body temperature, increased aggressive behavior, and disruption of learning.

 Grapefruit seed extract is an effective additive for cleaning and whitening toilets, sinks, tubs, tiles, carpets, laundry, clothing, linens, dish water and eating utensils. Many hospitals use grapefruit seed extract for these purposes with superior results.

 A growing number hospitals are now using grapefruit seed extract as a safe alternative to the harsh and damaging chemical based cleaners of the past. Grapefruit seed extract is being used in the wash to insure that linens are fungi and bacteria free. It also eliminates molds and odors associated with rancid laundry.

 Jerry Skidmore, C.L.M Manager of laundry operations for the Florida Hospital, wrote, "I have had 30 years experience in the laundry industry and it is only since using (Grapefruit Seed Extract) that I have had the peace of mind and assurance that the patients in our hospital and the other hospitals we serve have complete protection from fungal and bacteria infections that can be associated with linens. It is especially gratifying to know that even after many hours of exposure to the various bacteria that are always present in hospitals, our linens has been tested and found free of all harmful or pathogenic organisms."

 Hospitals also add grapefruit seed extract to their carpet shampooing machines. It is reported that just 10-15 drops of grapefruit seed extract per reservoir gallon is effective against many pathogenic organisms.

 Grapefruit seed extract is also a natural nontoxic wash and preservative for meats, fish, poultry, fruits, berries, vegetables and salads etc. It is used with water to wash fruits and vegetables to remove all pesticides, microbes and bacteria that may be present. Grapefruit seed extract leaves salad greens and vegetables clean crisp and ready to enjoy! With no after taste, a very important benefit!

 Grapefruit seed extract has been tested and proven by universities and countless laboratories throughout the world to be at least 10 times more effective than Colloidal Silver, Iodine and Clorox Bleach. Without the toxicity and high cost of commercial household cleaners.

 Air Conditioners / Air Purifiers: Filters harbor germs. Mix (?) drops grapefruit seed extract in 2-cups water. Spray filter elements once a week. Allow to dry before using. Cleaning these on a regular basis can help reduce the dangers of airborne mold, mildew, viruses etc.
 Carpets And Rugs: Mix (?) drops grapefruit seed extract per gallon of hot water. Grapefruit seed extract has been proven by hospitals to be extremely effective for this type of cleaning.
 Canned Foods: Grapefruit seed extract can be used to spray the tops of any canned foods before opening to decontaminate. Mix (?) drops grapefruit seed extract to 16oz (2 cups water). Spray on (let stand for 15-30 seconds) and rinse with clean water.
 Cutting Board Cleaner: Apply (?) drops grapefruit seed extract to cutting board and work in with a damp sponge or dishcloth. Let stand 30 minutes, rinse. This will remove salmonella, e-coli and many other harmful parasites and fungi.
 Dishes and Utensil Cleaner: Add (?) drops grapefruit seed extract to a sink full of dishwashing water or to final rinse. Add (?) drops grapefruit seed extract to automatic dishwashing detergent or in the final rinse. For sparkling clean sterilized dishes.
 Eyeglass Cleaner:Mix (?) drops grapefruit seed extract to 16oz (2 cups water). You can add more grapefruit seed extract for tough jobs.
 Floor Cleaner: Kitchen & bathroom. Use either normal or super strength mixes (listed Below) under regular or super strength cleaner.
 Furniture Cleaner Spray: Mix (?) drops grapefruit seed extract per 2-cups water and spray untill moist and let dry. Spray test a small area if you are worried about possible discoloration?
 Health Care Givers: Use grapefruit seed extract for cleaning the hands, before or after contact with the patient. Put (?) drops of extract directly on wet hands and rub it in. Grapefruit seed extract is used in hospitals to sterilize operating rooms surgical tools and as a presurgical scrub, as well.
 Hot Tubs and Jacuzzis: Mix (?) grapefruit seed extract for every 27 USA gallons of water or 100 liters. It will prevent the growth of algae and germs. Grapefruit seed extract isn't decomposed by UV rays and has no harmful vapors to rise up in the air you breath. Not only is possible damage avoided-good health is promoted.
 Hot Tubs Cleaning: Add (?) drops grapefruit seed extract to 1 qt water. Spray or wipe surfaces and surrounding areas.
 Humidifiers and Dehumidifiers: Mix (?) drops grapefruit seed extract per gallon of water to prevent contamination or growth of algae.
 Laundry Cleaner: Grapefruit seed extract is used by hospitals in the final rinse to sterilize linens and towels etc. Add (?) drops grapefruit seed extract to a small load of laundry or (?) drops to a large wash load. It may be added at the same time as laundry detergent. For best results add during the final rinse cycle.
 Paint Additive: Add (?) drops grapefruit seed extract per gallon (more can be added, but may soften the film slightly). Use more only when fungus or mildew threat is severe. Can be used with, primer coats, finish coats, deck stain and sealers, clear coating and specialty finishes like STO (trowelable finish system). It's particularly effective in areas of high humidity (restrooms, shower areas and all interior paint jobs). Can also be added to any patching, caulking or filling compounds to improve resistance to mold, mildew, fungal or other contamination at the same rate as above.
 Regular Strength Cleaner: For general cleaning. It can be sprayed on all surfaces around the home or office. To mix use (?) drops grapefruit seed extract in 2-cups water. For larger quantities mix (?) grapefruit seed extract per gallon of water. Grapefruit seed extract also whitens sinks, tubs, tiles and can be sprayed on algae.
 Soap Additive: Mix (?) drops grapefruit seed extract to 100ml (3.5 fluid oz). Add to any liquid hand or dish soap, shampoo or other personal hygiene products.
 Soap Making: Cosmetics/Soapmaking. Grapefruit seed extract improves quality and shelf life, using 0.05%, up to 1% solution.
 Sparkling Glassware / Dishes:Add (?) drops grapefruit seed extract to sinkful of wash or rinse water, or to automatic dishwasher. Grapefruit seed extract can be added to wash or rinse cycle of either wash method.
 Super Strength Cleaner: This can be used for more extreme jobs; toilet bowls, pet mess clean up etc. To mix use (?) drops grapefruit seed extract in 2-cups water. For larger quantities mix (?) grapefruit seed extract per gallon of water.
 Tar and Grease Remover: Grapefruit seed extract is good for breaking down tar and grease and other gummy substances. Use it undiluted and test a small area first as grapefruit seed extract has a bleaching effect.
 Veggie, Fruit, Meat and fish Cleaner : Contamination is a real concern with more and more fruits and vegetables being imported daily. Read more about imported fruits and vegetables. For sink washing; Add (?) or more drops grapefruit seed extract to a sink full of cold water and briefly soak vegetables, fruits, salads, meats, or poultry to remove all pesticides, microbes, and bacteria that may be present. For spray washing; add (?) drops grapefruit seed extract in 2-cups water and spray vegetables (or you may pour this solution into a small bowl and place lettuce or other vegetables including meat into the mix and submerse.) The latter method is by far the most effective. Let vegetables remain in this solution for a few minutes before rinsing for best results. You will be surprised at all of the dirt and grime etc. that grapefruit seed extract will remove. Sometimes the wash water is so dirty it looks like milk. This mix can also be used to extend the shelf life of all vegetables and fruits by 3-4 times, especially berries.
 Water Treatment: For emergency use or for long term storage. First water should be filtered. At the very least let the suspended particles settle. Retain the clear water. Add (?) drops grapefruit seed extract or (?) drops grapefruit seed extract per gallon of water. Depending on water quality and storage time. Up to (?) drops grapefruit seed extract per cup can be added in extreme cases. Shake or stir vigorously and let sit and settle for a few minutes before drinking.
 Wax Removal: Grapefruit seed extract seems to remove the wax that is sprayed on apples. Mix (?) drops grapefruit seed extract to 16oz (2 cups water). Spray a few times wipe and rub apples a little to help remove wax. Or wet apples and apply 1 drop grapefruit seed extract and a few drops of water per apple and rub with fingertips until apple lathers up and let sit for a few minutes before rinsing off. This seems to work much better than spraying.
 Window Cleaner: Mix (?) drops grapefruit seed extract in 2-cups water for larger amounts mix (?) drops more or less per gallon for sparkling clean glass.
Up.

  Alkalizing the blood:

 Another important benefit of grapefruit seed extract is that it is helpful in alkalizing the blood. Alkalizing the body fluids (raising pH) is one of the single most important health regeneration benefits available as disease causing microforms (fungi, bacteria, virus etc.) cannot survive in an alkaline oxygenated environment. Though lemons, limes, and grapefruits are chemically acid, tests show that when they are metabolized in the body they actually have an alkalizing effect. They have very little sugar and they contain an abundance of oxygen. This is a plus for anyone fighting candida or any other disease. Conclusion: Eat more fresh vegetables and less processed foods as most all vegetables are alkaline forming in the body.

 Grapefruit seed extract is effective at very low concentrations. During 1989-90, an international research team (reported in the Journal of Orthomolecular Medicine, Volume 5, No. 3, USA, 1990) examined the effects of GSE and compared this with 30 effective antibiotics and 18 proven fungicides. GSE was found to perform as well as any and all of the tested agents. Without the harsh side effects. It is a natural alternative to strong antibiotics.
 Grapefruit seed extract is nontoxic. According to an independent laboratory testing "Acute Oral Toxicity", it would take at least 4,000 times the normal dose of gse to produce a 50% chance of poisoning (called LD50).
 Because grapefruit seed extract does not exhibit the harsh side effects and high cost of pharmaceuticals it is becoming the alternative health choice for naturopathic physicians, clinics and the general public.

  Candida Yeast Infections & Parasitic Infections:

 There's something unique about this particular substance (grapefruit seed extract). Whatever it does, it does without the debilitating side effects. It has the advantage of being very safe. In the treatment of candidiasis it appears as effective as nystatin, caprylic acid, and other non-absorbed intestinal antifungal agents.
 Dr. Galland considers the availability of grapefruit seed extract to be "...a major therapeutic breakthrough for patients with chronic parasitic and yeast infections..."

 Many drug sensitive individuals find GSE to be much better tolerated than other antifungal preparations, and I have several patients in whom this product alone helped control chronic candidiasis when no other medication was tolerated or effective. In a test of 20 patients with vaginal yeast infection, 15 had relief of their symptoms in only 3 days after douching with grapefruit seed extract every 12 hours.
Up.

  Effects of 33% grapefruit extract on the growth of the yeast--like fungi, dermatopytes and moulds:Wiad Parazytol. 2001;47(4):845-9.Krajewska-Kulak E, Lukaszuk C, Niczyporuk W. Zaklad Teorii Pielegniarstwa Akademii Medycznej w Bialystoku, 15-096 Bialystok, ul. M. C. Sklodowskiej 7a.

 Grapefruit seed extract was discovered by Jacob Harich an american immunologist in 1980. Assessment of the influence of grapefruit extract on the yeast-like fungi strains--Candida albicans growth. Material used in this investigation was ATCC test Candida albicans strains no 10231, 200 of Candida albicans strains, 5 of Candida sp. strains isolated from patients with candidiasis symptoms from different ontocenosis and 12 of dermatophytes and moulds isolated from patients. The susceptibility of the Candida was determined by serial dilution method. It seems that 33% grapefruit extract exert a potent antifungal activity against the yeast like fungi strains and had low activity against dermatophytes and moulds. Further studies in vitro and in vivo on greater number of the yeast-like fungi strains and other fungi species are needed.

  Development and validation of an HPLC/UV/MS method for simultaneous determination of 18 preservatives in grapefruit seed extract.:J Agric Food Chem. 2006 May 31;54(11):3768-72.Ganzera M, Aberham A, Stuppner H.Institute of Pharmacy, University of Innsbruck, Innrain 52, 6020 Innsbruck, Austria. markus.ganzera@uibk.ac.at

 Grapefruit seed extracts are used in cosmetics, food supplements, and pesticides because of their antimicrobial properties, but suspicions about the true nature of the active compounds arose when synthetic disinfectants such as benzethonium or benzalkonium chloride were found in commercial products. The HPLC method presented herein allows the quality assessment (qualitative and quantitative) of these products for the first time. On the basis of a standard mixture of 18 preservatives most relevant for food and grapefruit products, a method was developed allowing the baseline separation of all compounds within 40 min. Optimum results were obtained with a C-8 stationary phase and a solvent system comprising aqueous trifluoroacetic acid, acetonitrile, and 2-propanol. The assay was fully validated and shown to be sensitive (LOD < or= 12.1 ng on-column), accurate (recovery rates > or = 96.1%), repeatable (sigma(rel) < or = 3.5%), precise (intra-day variation < or = 4.5%, interday variation < or = 4.1%), and rugged. Without any modifications the method could be adopted for LC-MS experiments, where the compounds of interest were directly assignable in positive ESI mode. The quantitative results of several products for ecofarming confirmed previous studies, as seven out of nine specimens were adulterated with preservatives in varying composition. The samples either contained benzethonium chloride (2.5-176.9 mg/mL) or benzalkonium chloride (138.2-236.3 mg/mL), together with smaller amounts of 4-hydroxybenzoic acid esters, benzoic acid, and salicylic acid.

  Grapefruit-seed extract attenuates ethanol-and stress-induced gastric lesions via activation of prostaglandin, nitric oxide and sensory nerve pathways.:World J Gastroenterol. 2005 Nov 7;11(41):6450-8.Brzozowski T, Konturek PC, Drozdowicz D, Konturek SJ, Zayachivska O, Pajdo R, Kwiecien S, Pawlik WW, Hahn EG.Department of Physiology, Jagiellonian University Medical College, Cracow, Poland. mpbrzozo@cyf-kr.edu.pl

 AIM: Grapefruit-seed extract (GSE) containing flavonoids, possesses antibacterial and antioxidative properties but whether it influences the gastric defense mechanism and gastroprotection against ethanol- and stress-induced gastric lesions remains unknown. METHODS: We compared the effects of GSE on gastric mucosal lesions induced in rats by topical application of 100% ethanol or 3.5 h of water immersion and restraint stress (WRS) with or without (A) inhibition of cyclooxygenase (COX)-1 activity by indomethacin and rofecoxib, the selective COX-2 inhibitor, (B) suppression of NO-synthase with L-NNA (20 mg/kg ip), and (C) inactivation by capsaicin (125 mg/kg sc) of sensory nerves with or without intragastric (ig) pretreatment with GSE applied 30 min prior to ethanol or WRS. One hour after ethanol and 3.5 h after the end of WRS, the number and area of gastric lesions were measured by planimetry, the gastric blood flow (GBF) was assessed by H2-gas clearance technique and plasma gastrin levels and the gastric mucosal generation of PGE2, superoxide dismutase (SOD) activity and malonyldialdehyde (MDA) concentration, as an index of lipid peroxidation were determined. RESULTS: Ethanol and WRS caused gastric lesions accompanied by the significant fall in the GBF and SOD activity and the rise in the mucosal MDA content. Pretreatment with GSE (8-64 mg/kg i g) dose-dependently attenuated gastric lesions induced by 100% ethanol and WRS; the dose reducing these lesions by 50% (ID50) was 25 and 36 mg/kg, respectively, and this protective effect was similar to that obtained with methyl PGE2 analog (5 microg/kg i g). GSE significantly raised the GBF, mucosal generation of PGE2, SOD activity and plasma gastrin levels while attenuating MDA content. Inhibition of PGE2 generation with indomethacin or rofecoxib and suppression of NO synthase by L-NNA or capsaicin denervation reversed the GSE-induced protection and the accompanying hyperemia. Co-treatment of exogenous calcitonine gene-related peptide (CGRP) with GSE restored the protection and accompanying hyperemic effects of GSE in rats with capsaicin denervation. CONCLUSION: GSE exerts a potent gastroprotective activity against ethanol and WRS-induced gastric lesions via an increase in endogenous PG generation, suppression of lipid peroxidation and hyperemia possibly mediated by NO and CGRP released from sensory nerves.

  Identification of benzalkonium chloride in commercial grapefruit seed extracts.:J Agric Food Chem. 2005 Sep 21;53(19):7630-6.Takeoka GR, Dao LT, Wong RY, Harden LA.Western Regional Research Center, Agricultural Research Service, US Department of Agriculture, Albany, California 94710, USA. grt@pw.usda.gov

 Commercial grapefruit seed extracts (GSE) were extracted with chloroform. The solvent was evaporated, and the resulting solid was subsequently analyzed by high-performance liquid chromatography (HPLC), electrospray ionization mass spectrometry (ESI/MS), tandem mass spectrometry (ESI/MS/MS), and elemental analysis (by proton-induced X-ray emission analysis). Three major constituents were observed by HPLC and were identified as benzyldimethyldodecylammonium chloride, benzyldimethyltetradecylammonium chloride, and benzyldimethylhexadecylammonium chloride. This mixture of homologues is commonly known as benzalkonium chloride, a widely used synthetic antimicrobial ingredient used in cleaning and disinfection agents.

  The effectiveness of grapefruit (Citrus paradisi) seeds in treating urinary tract infections.:J Altern Complement Med. 2005 Apr;11(2):369-71.Oyelami OA, Agbakwuru EA, Adeyemi LA, Adedeji GB.Department of Paediatrics and Child Health, Obafemi Awolowo University, Ile Ife, Osun State, Nigeria. tinaude_ogunlesi@yahoo.co.uk

 Three middle-aged males and one female were diagnosed as having urinary tract infections (UTIs) between 2001 and 2003 in the Wesley Guild Hospital, Ilesa, a unit of Obafemi Awolowo University Teaching Hospitals Complex, Ile Ife, Osun State, Nigeria. Of the 4 patients, only the female was asymptomatic. The 3 males had Pseudomonas aeruginosa, Klebsiella species, and Staphylococcus aureus, respectively, in their urine samples, while the female had Escherichia coli. All 4 patients were treated with grapefruit seeds (Citrus paradisi) orally for 2 weeks and they all responded satisfactorily to the treatment except the man with P. aeruginosa isolate. However, the initial profuse growth of Pseudomonas isolate in the patient that was resistant to gentamicin, tarivid, and augmentin later subsided to mild growth with reversal of the antibiotic resistance pattern after 2 weeks' treatment with grapefruit seeds. These preliminary data thus suggest an antibacterial characteristic of dried or fresh grapefruit seeds (C. paradisi) when taken at a dosage of 5 to 6 seeds every 8 hours, that is comparable to that of proven antibacterial drugs.
Up.

  Antimicrobial activity of grapefruit seed and pulp ethanolic extract.:Acta Pharm. 2004 Sep;54(3):243-50.Cvetnic Z, Vladimir-Knezevic S.Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia. zcvetnic@yahoo.com

 Antibacterial and antifungal activity of ethanolic extract of grapefruit (Citrus paradisi Macf., Rutaceae) seed and pulp was examined against 20 bacterial and 10 yeast strains. The level of antimicrobial effects was established using an in vitro agar assay and standard broth dilution susceptibility test. The contents of 3.92% of total polyphenols and 0.11% of flavonoids were determined spectrometrically in crude ethanolic extract. The presence of flavanones naringin and hesperidin in the extract was confirmed by TLC analysis. Ethanolic extract exibited the strongest antimicrobial effect against Salmonella enteritidis (MIC 2.06%, m/V). Other tested bacteria and yeasts were sensitive to extract concentrations ranging from 4.13% to 16.50% (m/V).

  Antioxidant activity of Citrus paradisi seeds glyceric extract.:Fitoterapia. 2004 Mar;75(2):221-4.Giamperi L, Fraternale D, Bucchini A, Ricci D.Istituto di Botanica e Orto Botanico Pierina Scaramella, Facolta di Farmacia, Universita degli Studi di Urbino, Via Bramante 28, 61029, Urbino, Italy.

 The antioxidant activity of Citrus paradisi (grapefruit) seeds glyceric extract dissolved in ethanol and in aqueous media was evaluated using three different methods: evaluation by DPPH assay, by 5-lipoxygenase assay and by luminol/xanthine/xanthine oxidase chemiluminescence assay. The total phenolic content was determined by the Prussian Blue method opportunely modified. The grapefruit seeds glyceric extract utilized as aqueous solutions demonstrated antioxidant properties better than those displayed by alcoholic solutions.

  Analysis of components in natural food additive "grapefruit seed extract" by HPLC and LC/MS:Eisei Shikenjo Hokoku. 1996;(114):38-42.Sakamoto S, Sato K, Maitani T, Yamada T.

 The components in a commercial natural food additive "Grapefruit seed extract" and the ethanol extract of grapefruit seeds were analyzed by HPLC and LC/MS. The HPLC chromatogram of the commercial grapefruit seed extract was quite different from that of the ethanol extract of grapefruit seeds. Three main peaks were observed in the chromatogram of the commercial grapefruit seed extract. By comparison of the retention times and the absorption spectra with those of authentic samples, two peaks were ascribed to methyl-p-hydroxybenzoate and 2,4,4'-trichloro-2'-hydroxydiphenylether (triclosan). Triclosan was also identified by LC/MS by using the negative electrospray ionization method.
Up.

  Development and validation of an HPLC/UV/MS method for simultaneous determination of 18 preservatives in grapefruit seed extract.:

 Grapefruit seed extracts are used in cosmetics, food supplements, and pesticides because of their antimicrobial properties, but suspicions about the true nature of the active compounds arose when synthetic disinfectants such as benzethonium or benzalkonium chloride were found in commercial products. The HPLC method presented herein allows the quality assessment (qualitative and quantitative) of these products for the first time. On the basis of a standard mixture of 18 preservatives most relevant for food and grapefruit products, a method was developed allowing the baseline separation of all compounds within 40 min. Optimum results were obtained with a C-8 stationary phase and a solvent system comprising aqueous trifluoroacetic acid, acetonitrile, and 2-propanol. The assay was fully validated and shown to be sensitive (LOD < or= 12.1 ng on-column), accurate (recovery rates > or = 96.1%), repeatable (sigma(rel) < or = 3.5%), precise (intra-day variation < or = 4.5%, interday variation < or = 4.1%), and rugged. Without any modifications the method could be adopted for LC-MS experiments, where the compounds of interest were directly assignable in positive ESI mode. The quantitative results of several products for ecofarming confirmed previous studies, as seven out of nine specimens were adulterated with preservatives in varying composition. The samples either contained benzethonium chloride (2.5-176.9 mg/mL) or benzalkonium chloride (138.2-236.3 mg/mL), together with smaller amounts of 4-hydroxybenzoic acid esters, benzoic acid, and salicylic acid.

  Grapefruit-seed extract attenuates ethanol-and stress-induced gastric lesions via activation of prostaglandin, nitric oxide and sensory nerve pathways.:

 Aim Grapefruit-seed extract (GSE) containing flavonoids, possesses antibacterial and antioxidative properties but whether it influences the gastric defense mechanism and gastroprotection against ethanol- and stress-induced gastric lesions remains unknown. Methods: We compared the effects of GSE on gastric mucosal lesions induced in rats by topical application of 100% ethanol or 3.5 h of water immersion and restraint stress (WRS) with or without (A) inhibition of cyclooxygenase (COX)-1 activity by indomethacin and rofecoxib, the selective COX-2 inhibitor, (B) suppression of NO-synthase with L-NNA (20 mg/kg ip), and (C) inactivation by capsaicin (125 mg/kg sc) of sensory nerves with or without intragastric (ig) pretreatment with GSE applied 30 min prior to ethanol or WRS. One hour after ethanol and 3.5 h after the end of WRS, the number and area of gastric lesions were measured by planimetry, the gastric blood flow (GBF) was assessed by H2-gas clearance technique and plasma gastrin levels and the gastric mucosal generation of PGE2, superoxide dismutase (SOD) activity and malonyldialdehyde (MDA) concentration, as an index of lipid peroxidation were determined. Results: Ethanol and WRS caused gastric lesions accompanied by the significant fall in the GBF and SOD activity and the rise in the mucosal MDA content. Pretreatment with GSE (8-64 mg/kg i g) dose-dependently attenuated gastric lesions induced by 100% ethanol and WRS; the dose reducing these lesions by 50% (ID50) was 25 and 36 mg/kg, respectively, and this protective effect was similar to that obtained with methyl PGE2 analog (5 microg/kg i g). GSE significantly raised the GBF, mucosal generation of PGE2, SOD activity and plasma gastrin levels while attenuating MDA content. Inhibition of PGE2 generation with indomethacin or rofecoxib and suppression of NO synthase by L-NNA or capsaicin denervation reversed the GSE-induced protection and the accompanying hyperemia. Co-treatment of exogenous calcitonine gene-related peptide (CGRP) with GSE restored the protection and accompanying hyperemic effects of GSE in rats with capsaicin denervation. Conclusion: GSE exerts a potent gastroprotective activity against ethanol and WRS-induced gastric lesions via an increase in endogenous PG generation, suppression of lipid peroxidation and hyperemia possibly mediated by NO and CGRP released from sensory nerves.

  Extract of grapefruit-seed reduces acute pancreatitis induced by ischemia/reperfusion in rats: possible implication of tissue antioxidants.:

 Grapefruit seed extract (GSE) has been shown to exert antibacterial, antifungal and antioxidant activity possibly due to the presence of naringenin, the flavonoid with cytoprotective action on the gastric mucosa. No study so far has been undertaken to determine whether this GSE is also capable of preventing acute pancreatic damage induced by ischemia/reperfusion (I/R), which is known to result from reduction of anti-oxidative capability of pancreatic tissue, and whether its possible preventive effect involves an antioxidative action of this biocomponent. In this study carried out on rats with acute hemorrhagic pancreatitis induced by 30 min partial pancreatic ischemia followed by 6 h of reperfusion, the GSE or vehicle (vegetable glycerin) was applied intragastrically in gradually increasing amounts (50-500 microl) 30 min before I/R. Pretreatment with GSE decreased the extent of pancreatitis with maximal protective effect of GSE at the dose 250 microl. GSE reduced the pancreatitis-evoked increase in serum lipase and poly-C specific ribonuclease activity, and attenuated the marked fall in pancreatic blood flow and pancreatic DNA synthesis. GSE administered alone increased significantly pancreatic tissue content of lipid peroxidation products, malondialdehyde and 4-hydroxyalkens, and when administered before I/R, GSE reduced the pancreatitis-induced lipid peroxidation. We conclude that GSE exerts protective activity against I/R-induced pancreatitis probably due to the activation of antioxidative mechanisms in the pancreas and the improvement of pancreatic blood flow.

  Antimicrobial activity of grapefruit seed and pulp ethanolic extract.:

 Antibacterial and antifungal activity of ethanolic extract of grapefruit (Citrus paradisi Macf., Rutaceae) seed and pulp was examined against 20 bacterial and 10 yeast strains. The level of antimicrobial effects was established using an in vitro agar assay and standard broth dilution susceptibility test. The contents of 3.92% of total polyphenols and 0.11% of flavonoids were determined spectrometrically in crude ethanolic extract. The presence of flavanones naringin and hesperidin in the extract was confirmed by TLC analysis. Ethanolic extract exibited the strongest antimicrobial effect against Salmonella enteritidis (MIC 2.06%, m/V). Other tested bacteria and yeasts were sensitive to extract concentrations ranging from 4.13% to 16.50% (m/V).

  Analysis of components in natural food additive "grapefruit seed extract" by HPLC and LC/MS:

 The components in a commercial natural food additive "Grapefruit seed extract" and the ethanol extract of grapefruit seeds were analyzed by HPLC and LC/MS. The HPLC chromatogram of the commercial grapefruit seed extract was quite different from that of the ethanol extract of grapefruit seeds. Three main peaks were observed in the chromatogram of the commercial grapefruit seed extract. By comparison of the retention times and the absorption spectra with those of authentic samples, two peaks were ascribed to methyl-p-hydroxybenzoate and 2,4,4'-trichloro-2'-hydroxydiphenylether (triclosan). Triclosan was also identified by LC/MS by using the negative electrospray ionization method.
Up.

  Identification of benzalkonium chloride in commercial grapefruit seed extracts.:

 Commercial grapefruit seed extracts (GSE) were extracted with chloroform. The solvent was evaporated, and the resulting solid was subsequently analyzed by high-performance liquid chromatography (HPLC), electrospray ionization mass spectrometry (ESI/MS), tandem mass spectrometry (ESI/MS/MS), and elemental analysis (by proton-induced X-ray emission analysis). Three major constituents were observed by HPLC and were identified as benzyldimethyldodecylammonium chloride, benzyldimethyltetradecylammonium chloride, and benzyldimethylhexadecylammonium chloride. This mixture of homologues is commonly known as benzalkonium chloride, a widely used synthetic antimicrobial ingredient used in cleaning and disinfection agents.

  The effectiveness of grapefruit (Citrus paradisi) seeds in treating urinary tract infections.:

 Three middle-aged males and one female were diagnosed as having urinary tract infections (UTIs) between 2001 and 2003 in the Wesley Guild Hospital, Ilesa, a unit of Obafemi Awolowo University Teaching Hospitals Complex, Ile Ife, Osun State, Nigeria. Of the 4 patients, only the female was asymptomatic. The 3 males had Pseudomonas aeruginosa, Klebsiella species, and Staphylococcus aureus, respectively, in their urine samples, while the female had Escherichia coli. All 4 patients were treated with grapefruit seeds (Citrus paradisi) orally for 2 weeks and they all responded satisfactorily to the treatment except the man with P. aeruginosa isolate. However, the initial profuse growth of Pseudomonas isolate in the patient that was resistant to gentamicin, tarivid, and augmentin later subsided to mild growth with reversal of the antibiotic resistance pattern after 2 weeks' treatment with grapefruit seeds. These preliminary data thus suggest an antibacterial characteristic of dried or fresh grapefruit seeds (C. paradisi) when taken at a dosage of 5 to 6 seeds every 8 hours, that is comparable to that of proven antibacterial drugs.

  Interaction of grapefruit juice and calcium channel blockers.:

 Drug-drug interactions are commonly recognized occurrences in the hypertensive population. Drug-nutrient interactions, however, are less well appreciated. The grapefruit juice-calcium channel blocker interaction is one that has been known since 1989. The basis for this interaction has been diligently explored and appears to relate to both flavanoid and nonflavanoid components of grapefruit juice interfering with enterocyte CYP3A4 activity. In the process, presystemic clearance of susceptible drugs decreases and bioavailability increases. A number of calcium channel blockers are prone to this interaction, with the most prominent interaction occurring with felodipine. The calcium channel blocker and grapefruit juice interaction should be incorporated into the knowledge base of rational therapeutics for the prescribing physician.

  Design, synthesis and evaluation of furanocoumarin monomers as inhibitors of CYP3A4.:

 A number of furanocoumarins isolated from grapefruit juice have been found to inhibit CYP3A4 activity in vitro. In this study, we have designed and synthesised a range of analogues based on bergamottin to investigate the relationship between chemical structure and inhibition of CYP3A4 activity. Studies were performed using human liver microsomes and human intestinal S9 fraction, with testosterone as the marker substrate. With the exception of the coumarin and phenolic furanocoumarin derivatives, which were inactive, the alkyloxy-furanocoumarin analogues were found to inhibit CYP3A4 activity in a dose dependent manner, with observed IC50 values ranging from 0.13 +/- 0.03 to 49.3 +/- 1.9 microM. The unsaturated furan derivatives were found to exhibit time-dependent inhibition, showing a 2-, 4- and 14-fold increase in potency for 6',7'-epoxybergamottin, 6',7'-dihydroxybergamottin and bergamottin, respectively after a preincubation period of ten minutes. Reduction of the furan moiety resulted in an 11-fold decrease in inhibitory potency, suggesting that this functional group is key to the interaction between these compounds and CYP3A4.

  The effects of grapefruit on weight and insulin resistance: relationship to the metabolic syndrome.:

 To study the effects of grapefruit and grapefruit products on body weight and metabolic syndrome, 91 obese patients were randomized to either placebo capsules and 7 ounces (207 mL) of apple juice, grapefruit capsules with 7 ounces (207 mL) of apple juice, 8 ounces (237 mL) of grapefruit juice with placebo capsule, or half of a fresh grapefruit with a placebo capsule three times a day before each meal. Metabolic syndrome parameters were measured at the beginning and end of 12 weeks. After 12 weeks, the fresh grapefruit group had lost 1.6 kg, the grapefruit juice group had lost 1.5 kg, the grapefruit capsule group had lost 1.1 kg, and the placebo group had lost 0.3 kg. The fresh grapefruit group lost significantly more weight than the placebo group (P < .05). A secondary analysis of those with the metabolic syndrome in the four treatment groups demonstrated a significantly greater weight loss in the grapefruit, grapefruit capsule, and grapefruit juice groups compared with placebo (P < .02). There was also a significant reduction in 2-hour post-glucose insulin level in the grapefruit group compared with placebo. Half of a fresh grapefruit eaten before meals was associated with significant weight loss. In metabolic syndrome patients the effect was also seen with grapefruit products. Insulin resistance was improved with fresh grapefruit. Although the mechanism of this weight loss is unknown it would appear reasonable to include grapefruit in a weight reduction diet.

  Furocoumarins from grapefruit juice and their effect on human CYP 3A4 and CYP 1B1 isoenzymes.:

 Bioactive compounds present in grapefruit juice are known to increase the bioavailability of certain medications by acting as potent CYP 3A4 inhibitors. An efficient technique has been developed for isolation and purification of three furocoumarins. The isolated compounds have been tested for the inhibition of human CYP 1B1 isoform using specific substrates. Grapefruit juice was extracted with ethyl acetate (EtOAc) and the dried extract was loaded onto silica gel column chromatography. Further, column fractions were subjected to preparative HPLC to obtain three compounds. The purity of these compounds was analyzed by HPLC and structures were determined by NMR studies. The identified compounds, bergamottin, 6',7'-dihydroxybergamottin (DHB), and paradisin-A, were tested for their inhibitory effects on hydroxylase and O-dealkylase activities of human cytochrome P450 isoenzymes CYP 3A4 and CYP 1B1. Paradisin-A was found to be a potent CYP 3A4 inhibitor with an IC50 of 1.2 microM followed by DHB and bergamottin. All three compounds showed a substantial inhibitory effect on CYP 3A4 below 10 microM. Inhibitory effects on CYP 1B1 exhibited a greater variation due to the specificity of substrates. Paradisin A showed an IC50 of 3.56+/-0.12 microM for the ethoxy resorufin O-dealkylase (EROD) activity and 33.56+/-0.72 microM for the benzyloxy resorufin (BROD). DHB and bergamottin showed considerable variations for EROD and BROD activities with an IC50 of 7.17 microM and 13.86 microM, respectively.

  Grapefruit consumption improves vitamin C status in periodontitis patients.:

 Objective: Previous studies demonstrate a relationship between a lack of vitamin C and increased risk of periodontal disease. In the present study we examine the vitamin C plasma levels and inflammatory measures in periodontitis patients before and after the consumption of grapefruit. SUBJECTS AND METHODS: Fifty-eight patients with chronic periodontitis were assigned to the test group (non-smokers n=21, smokers n=17) and a diseased control group (non-smokers n=11, smokers n=9). Furthermore, 22 healthy subjects were recruited to compare vitamin C plasma levels between periodontally diseased and healthy subjects. Clinical evaluations, including plaque index (PI), sulcus bleeding index (SBI), probing pocket depths (PPD) and plasma vitamin C levels, were performed at baseline, and after two weeks of grapefruit consumption. RESULTS: At baseline, we observed significantly reduced plasma vitamin C levels in the test group and diseased controls in comparison with the healthy controls. On principle, smokers showed lower levels of vitamin C (mean 0.39 +/- 0.17 mg dl(-1)) than non-smokers (mean 0.56+/-0.29 mg dl(-1)). After grapefruit consumption, the mean plasma vitamin C levels rose significantly in the test group compared to the diseased controls (non-smokers: 0.87+/-0.39 mg dl(-1), smokers: 0.74+/-0.30 mg dl(-1)). Furthermore the SBI was reduced in the test group (non-smokers: from 1.68+/-0.6 to 1.05+/-0.6, p<0.001), whereas PI and PPD were unaffected. CONCLUSION: The present results show that periodontitis patients are characterised by plasma vitamin C levels below the normal range, especially in smokers. The intake of grapefruit leads to an increase in plasma vitamin C levels and improves sulcus bleeding scores. Longer term studies are necessary to determine whether other periodontal outcomes improve with such supplementation especially in smokers.
Up.

PrevNext

Reference:
  • 1.Grapefruit and GSE.Function and Application of Grapefruit seed extract.

♥The article and literature was edited by herbalist of MDidea Extracts Professional.It runs a range of online descriptions about the titled herb and related phytochemicals,including comprehensive information related,summarized updating discoveries from findings of herbalists and clinical scientists from this field.The electronic data information published at our official website www.mdidea.com and www.mdidea.net,we tried best to update it to latest and exact as possible.
♣ last edit date:

Available Product
  • Name:Grapefruit Seed Extract
  • Serie No:R011.
  • Specifications:Aqueous Extract 10:1,Ethanol Extract 10:1 TLC.
  • INCI Name:CITRUS GRANDIS SEED EXTRACT
  • EINECS/ELINCS No.:289-904-6
  • CAS:90045-43-5
  • Chem/IUPAC Name:Citrus Grandis Seed Extract is an extract of the seeds of the grapefruit,Citrus grandis,Rutaceae.
  • Other Names:Citrus Grandis Seed Extract,Grapefruit Extract,GSE,Citrus paradisi Macfad,Citrus Seed,Pummelo Semen,Pummelo Seed,Pummelo Kernel,You Zi Zi.

See Specification Details

Product Display
Grapefruit Seed Extract.

See Display Details