Botanical info and medicinal uses of Ecliptae Herba.

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Eclipta prostrata Quality control including chromatographic fingerprint profiling.

Ecliptae Prostratae Extract INCI Name Eclipta Prostrata Extract Eclipta prostrata Linn CAS 93165-22-1 EINECS ELINCS No 296-907-6 Han Lian Cao Herba Ecliptae Extract Verbesina alba Keremek Hutan photo picture image The plant material (325 g of leaves) was extracted by maceration with agitation, in methanol (2.0 L) by 24 hours. The solvent was removed with reduced pressure obtaining 70,4 g of residue. It was suspended in 600 ml of water and warmed in water bath at less than 75 Deg C for 30 min. Then, the aqueous phase was filtered and extracted with ethyl acetate (250 ml x 4), the fractions were dried giving 1,74 g of residue (Wagner et al., 1986).

 A thin layer chromatography of ethyl acetate fraction, on silica gel 60 F254, using as eluent chloroform-methanol (70:30) and sprayed with FeCl3/HCl 0,5 N, revealing a spot of green color (Wagner et al., 1986). The EtOAc fraction was separated on medium pressure column chromatography on silica gel 0,015 - 0,040 mm, using as mobile phase CHCl3-MeOH (90:10); (80:20) and (70:30), obtaining two compounds (Eca-1 and Eca-2). Compound Eca-1 was identified like wedelolactone (Zafar et al., 1999) based on its spectroscopic properties. This compound shows on TLC, a blue fluorescence under UV light, a green color is revealed with ferric chloride and a Rf: 0.64, in the solvent system chloroform-methanol (8:2). The compound Eca-2 was identified as a mixture of triterpenes. norwedelactona (Sagar et al., 2000) was detected in the sample under study in very low concentrations detectable only by NMR (Olmedo et al., 2004).

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citations1.Botanical info and medicinal uses of Ecliptae Herba.

last edit date:16th,June.2009.