Product Name:
Synonym:
Definition:Psoralea fruit Extract are majorly composed of
Chemical information disclosed as following table:
Research Update:Babchi seeds,Malaytea Scurfpea Fruit,Psoralea corylifolia.
Study on micro-wave processed of Psoralea corylifolia with uniform and regression analysis.:Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1167-70. Chinese.Guo YH, Jia TZ, Lin GM.Liaoning Traditional Chinese Medicine University, Shenyang 110032, China.
OBJECTIVE: To optimize the herb processing process for the total contains of psoralen and isopsoralen and the rate of extract in Psoralea corylifolia by micro-wave herb processing. METHOD: The contains of psoralen and isopsoralen was obtained by HPLC. The micro-wave herb processing process was optimized by the way of uniform design and contour map. RESULT: The optimum process was:20% as salt concentration, 4h as immerse time, micro-wave strength as strong, 270 seconds as micro-wave time. The absolute error of the predicted value from the models were smaller than 6% and 0. 3% respectively. CONCLUSION: The regression models are notable and reasonable, which can forecast results precisely.
Psoralea corylifolia Extract Ameliorates Experimental Osteoporosis in Ovariectomized Rats.:Am J Chin Med. 2007;35(4):669-80.Tsai MH, Huang GS, Hung YC, Bin L, Liao LT, Lin LW.Department of Management Information Systems, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan.
We evaluated the protective effect of Psoralea corylifolia L. (PCL) extract on the ovariectomized (OVX) rat model. The biochemical markers of bone turnover, calcium metabolism, and calcium balance were examined. PCL extract (25 mg or 50 mg/kg body weight/day) was orally administrated to OVX rats for 3 months. PCL extract did not alter weight gain or uterus weight in OVX rats. PCL extract significantly increased serum Ca (calcium) levels (p < 0.05, vs. OVX group) as well as decreased urinary Ca excretion (p < 0.05 vs. OVX group) in OVX rats. The upregulation of serum osteocalcin level by ovariectomy was suppressed by treatment with PCL extract in rats (p < 0.05, vs. OVX group). PCL extract increased bone mineral density at 50 mg/kg body weight/day in OVX rats (p < 0.05, vs. OVX group). Our results indicate that orally administrated PCL extract can decrease urinary calcium excretion and decrease serum osteocalcin in OVX rats, resulting in positive effects on bone mineral density as well as bone formation. In conclusion, our studies showed that PCL might be a potential candidate for treatment of postmenopausal osteoporosis.
Inhibitive effects of Fructus Psoraleae extract on dopamine transporter and noradrenaline transporter.:J Ethnopharmacol. 2007 Jul 25;112(3):498-506. Epub 2007 Apr 25.Zhao G, Li S, Qin GW, Fei J, Guo LH.Cell Star Bio-Technologies Co., Limited, 1 Jury Road, Shanghai 201203, People's Republic of China.
A petroleum ether extract (FP) from Fructus Psoraleae, seeds of Psoralea corylifolia L. (Leguminosae), was found to strongly inhibit dopamine (DA) uptake by dopamine transporter (DAT) heterogeneously expressed cells (D8 cells) and noradrenaline (NE) uptake by noradrenaline transporter (NET) heterogeneously expressed cells, which, however, had no effect on gamma-aminobutyric acid transporter heterogeneously expressed cells and serotonin transporter heterogeneously expressed cells at the concentration up to 100 microg/ml. These inhibitory effects were also confirmed by experiments on SK-N-SH cell line and synaptosomes from rats' brains. In addition, FP showed a significantly mitigating effect on 1-methyl-4-pyridinium induced injury of D8 cells. Meanwhile, FP dose-dependently reduced the binding of tritium-labeled cocaine analog (-)-2beta-carbomethoxy-3beta-(4-fluorophenyl) tropane to DAT of D8 cells, which suggests that FP may inhibit DAT activity in the same way as cocaine does. Behavioral study showed FP had a long-lasting stimulant effects on the activity of intact mice and reserpinized mice. So FP is proposed as a kind of DAT and NET inhibitor and may be involved in the process of regulating the DA and NE system, and FP or its unknown bioactive compounds may be developed into new medicines for disorders such as Parkinson's disease, depression, Attention Deficit Hyperactivity Disorder (ADHD) or cocaine addiction.
Psoralea corylifolia L. inhibits mitochondrial complex I and proteasome activities in SH-SY5Y cells.:Ann N Y Acad Sci. 2007 Apr;1100:486-96.Tang SY, Gruber J, Wong KP, Halliwell B.National University of Singapore, University Hall, Lee Kong Chian Wing, UHL 05-02G, 21 Lower Kent Ridge Road, Singapore. bchbh@nus.edu.sg
The growing interest in alternative medicines, including traditional medicinal plants, has caused some health concerns due to poor awareness in the general population of the possible side effects from inappropriate practices. Psoralea corylifolia L. has been used in Chinese and Indian traditional medicine for the treatment of various inflammatory diseases of the skin and to improve vitality. Our data show that the extract obtained from the seeds of Psoralea corylifolia L. decreased mitochondrial complex I and proteasome activities; and oxidative stress might be an early event.
Studies on the chemical constituents of Psoralea corylifolia L.:J Asian Nat Prod Res. 2007 Jan-Feb;9(1):41-4.Ruan B, Kong LY, Takaya Y, Niwa M.Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, China.
A new isoflavone, corylinin (1), along with six known compounds, isopsoralen (2), psoralen (3), sophoracoumestan A (4), neobavaisoflavone (5), daidzin (6) and uracil (7), have been isolated from the dried fruits of Psoralea corylifolia L. The structure of 1 was established as 7,4'-dihydroxy-3'-[(E)-3,7-dimethyl-2,6-octadienyl]isoflavone on the basis of the spectroscopic methods. Structures of the known compounds were identified by comparison of the literature.
Cyclobakuchiol C, a new bakuchiol derivative from Psoralea coryllfolia.:J Asian Nat Prod Res. 2007 Jan-Feb;9(1):29-33.Yin S, Fan CQ, Yue JM.State Key Laboratory of Drug Research, Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Zhangjiang Hi-Tech Park, Shanghai, China.
A new compound, cyclobakuchiol C (1), together with four known bakuchiol derivatives, 2-5, was isolated from the non-polar fraction of the seeds of Psoralea corylifolia, and compounds 3-5 were identified from this plant for the first time. The structure of 1 was determined by spectroscopic methods, especially 2D NMR experiments.
Bakuchiol-induced caspase-3-dependent apoptosis occurs through c-Jun NH2-terminal kinase-mediated mitochondrial translocation of Bax in rat liver myofibroblasts.:Eur J Pharmacol. 2007 Mar 22;559(2-3):115-23. Epub 2007 Jan 23.Park EJ, Zhao YZ, Kim YC, Sohn DH.Department of Pharmacy, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
Liver fibrosis and cirrhosis may be reversible, possibly through the selective clearance of activated hepatic stellate cells/myofibroblasts by apoptosis. Hepatic stellate cells transdifferentiate into myofibroblast-phenotype cells in culture, a process that recapitulates hepatic stellate cell activation in vivo. Bakuchiol, a prenylated phenolic terpene isolated from the seed of Psoralea corylifolia L. (Leguminosae), reduced activated hepatic stellate cells when treated to rats during liver injury recovery period as demonstrated by alpha-smooth muscle actin immunostaining in rat liver and induced apoptosis in activated hepatic stellate cells/myofibroblasts as demonstrated by DNA fragmentation, activation of caspase-3, release of cytochrome c into the cytoplasm, translocation of Bax into mitochondria, and the proteolytic cleavage of poly(ADP-ribose) polymerase (PARP) in vitro. Bakuchiol-induced apoptosis was prevented by z-DEVD-fmk, a specific inhibitor of caspase-3, and z-VAD-fmk, a general caspase inhibitor, suggesting that bakuchiol-induced apoptosis occurs through a caspase-3-dependent pathway in vitro. Bakuchiol treatment stimulated the activation of extracellular signal-regulated kinase 1/2 (ERK), c-Jun NH2-terminal protein kinase (JNK), and p38 mitogen-activated protein kinases (MAPK) in vitro. Pretreatment with SP600125 attenuated the bakuchiol-induced translocation of Bax into mitochondria, cytochrome c release into the cytosol, caspase-3 activation, and PARP cleavage. In contrast, preincubation with SB203580, a p38 MAPK inhibitor, and U0126, an ERK inhibitor, had no effect on bakuchiol-induced cell death and caspase-3 activity. Taken together, these findings indicate that bakuchiol induces caspase-3-dependent apoptosis through the activation of JNK, followed by Bax translocation into mitochondria in rat liver myofibroblasts.
Comparation of constitutions between raw Psoralea corylifolia L. and processed one.:Zhong Yao Cai. 2006 Nov;29(11):1142-4. Chinese.Guo YH, Luo ZD, Jia TZ.Department of Pharmaceutical College of Liaoning Traditional Chinese Medicine, Shenyang 110032, China. guoyanyualnu@sina.com
OBJECTIVE: To study the processing material foundation of Psoralea corylfolia L. and compare the content of psoralen, isopsoralen and microelement between raw Psoralea corylifolia L. and processed one. METHODS: The content of psoralen, isopsoralen and microelement were obtained by HPLC-gradient elution and ICP-AES. RESULTS: The content of psoralen, isopsoralen and microelement Mn, Ca, Mg, Fe, Zn were raising, but the change of that of microelement Cu was not obvious. CONCLUSION: The content of effective composition of Psoralea corylifolia L. is raised by Lei-Gong method and the biological activity is improved simultaneously.
Bioactive constituents from Chinese natural medicines. XX. Inhibitors of antigen-induced degranulation in RBL-2H3 cells from the seeds of Psoralea corylifolia.:Chem Pharm Bull. 2007 Jan;55(1):106-10.
The ethyl acetate-soluble fraction of the methanolic extract from the seeds of Psoralea corylifolia was found to inhibit the release of beta-hexosaminidase, as a marker of antigen-IgE-induced degranulation in RBL-2H3 cells. Sixteen constituents were isolated from the ethyl acetate-soluble fraction and several constituents, Delta(1),3-hydroxybakuchiol (IC(50) = 49 microM), Delta(3),2-hydroxybakuchiol (69 microM, bavachin (58 microM), and psoralidin (ca. 100 microM), showed inhibitory activities against the antigen-induced degranulation.
Behavioral and biochemical studies of total furocoumarins from seeds of Psoralea corylifolia in the chronic mild stress model of depression in mice.:Phytomedicine. 2007 Aug;14(7-8):523-9. Epub 2006 Nov 3.Chen Y, Wang HD, Xia X, Kung HF, Pan Y, Kong LD.State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Nanjing University, Nanjing 210093, People's Republic of China.
Depression is related to alterations of the monoamine oxidase (MAO), hypothalamic-pituitary-adrenal (HPA) axis, and oxidative systems, and some antidepressants achieve their therapeutic effects through alteration of following biochemical markers of depression: MAO-A and MAO-B activities, cortisol levels, superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels. The seeds of Psoralea corylifolia, otherwise known as Buguzhi, have long been used for treatments of various symptoms associated with aging in China. Furocoumarins are the most widespread secondary metabolites in this species. The present study was designed to evaluate the potential antidepressant-like activity of total furocoumarins of P. corylifolia (TFPC) in the chronic mild stress (CMS) model of depression. Mice subjected to CMS exhibited a reduction in sucrose intake. Conversely, brain MAO-A and MAO-B activities, plasma cortisol levels, and liver SOD activity and MDA levels were increased following CMS exposures. The time-course for reversal of CMS-induced deficits in sucrose consumption by TFPC was dose-dependent. Thus, the statistically significant effect of the higher dose of TFPC (50 mg/kg body wt.) was observed after 3 days of treatment, while 6 days of treatment were required in the group receiving a lower dose (30 mg/kg body wt.) of TFPC. TFPC reversed these biochemical changes. These results suggest that TFPC may possess potent and rapid antidepressant properties that are mediated via MAO, the HPA axis and oxidative systems and these antidepressant actions could make TFPC a potentially valuable drug for the treatment of depression in the elderly.
Isolation and structure identification of a new isoflavone from Psoralea corylifolias.:Yao Xue Xue Bao. 2006 Jan;41(1):76-9. Chinese.Yang TT, Qin MJ.Department of Resource Science of Chinese Medicines, China Pharmaceutical University, Nanjing 210038, China.
AIM: To study the chemical constituents of the fruits of Psoralea corylifolia L. METHODS: The constituents were separated and purified by column chromatography with normal phase silica gel and polyamide. Their structures were identified on the basis of physical and spectral data (MS, NMR, HMBC and HMQC). RESULTS: Six compounds were isolated and identified as psoralen (1), isopsoralen (2), bavachalcone (3), psoralidin (4), daidzin (5) and bavadin (6). CONCLUSION: Compound 6 is a new compound. Compound 5 was isolated from this plant for the first time.
Psoralenoside and isopsoralenoside, two new benzofuran glycosides from Psoralea corylifolia.:Chem Pharm Bull. 2006 May;54(5):714-6.Qiao CF, Han QB, Mo SF, Song JZ, Xu LJ, Chen SL, Yang DJ, Kong LD, Kung HF, Xu HX.Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong, P.R. China.
Two new benzofuran glycosides, called psoralenoside and isopsoralenoside, were isolated from the fruits of Psoralea corylifolia, together with nine known compounds. Their structures were elucidated by detailed spectral analyses including extensive two dimensional (2D) NMR spectra.
Psc-AFP, an antifungal protein with trypsin inhibitor activity from Psoralea corylifolia seeds.:Peptides. 2006 Jul;27(7):1726-31. Epub 2006 Mar 13.Yang X, Li J, Wang X, Fang W, Bidochka MJ, She R, Xiao Y, Pei Y.Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture, Biotechnology Research Center, Southwest University, Beibei, Chongqing 400716, PR China. yangxy@swau.edu.cn
An antifungal protein designated as Psc-AFP, with an apparent molecular mass of 18kDa, was isolated from a traditional Chinese herb, malaytea scurfpea (Psoralea corylifolia L.). The isolation procedure entailed extraction, cation exchange chromatography on CM FF, gel filtration chromatography on Superdex 75 and reversed-phase high performance liquid chromatography on SOURCE 5RPC column. Automated Edman degradation determined the partial N-terminal sequence of Psc-AFP to be NH2-EWEPVQNGGSSYYMVPRIWA, which displayed homology with plant trypsin inhibitors. The protease inhibitor activity of Psc-AFP was then confirmed by the inhibition on trypsin. Psc-AFP at 10 microM inhibited the mycelial growth of Alternari brassicae, Aspergillus niger, Fusarium oxysporum and Rhizoctonia cerealis, suggesting that Psc-AFP has a role in the defense against pathogens.
Prenylflavones from Psoralea corylifolia inhibit nitric oxide synthase expression through the inhibition of I-kappaB-alpha degradation in activated microglial cells.:Biol Pharm Bull. 2005 Dec;28(12):2253-7.Lee MH, Kim JY, Ryu JH.College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Korea.
The overproduction of nitric oxide (NO) by inducible nitric oxide synthase (iNOS) switches the function of NO from a physiological neuromodulator to a neurotoxic effector in central nervous system (CNS) after brain injury. From the methanol extracts of Psoralea corylifolia, we purified two inhibitors of NO production in lipopolysaccharide (LPS)-activated microglia by activity guided purification along with two inactive compounds. The active compounds were identified as a chromenoflavanone [7,8-dihydro-8-(4-hydroxyphenyl)-2,2-dimethyl-2H,6H-benzo-(1,2-b:5,4-b')dipyran-6-one] (1) and 4-hydroxylonchocarpin (2). And the inactive two compounds were identified as bavachinin (3) and bavachalcone (4) by spectral analysis. The compound 2 was isolated first time from this plant. Compounds 1 and 2 inhibited the production of NO in LPS-activated microglia in a dose dependent manner (IC(50)'s were 11.4, 10.2 microM, respectively). They also suppressed the expression of protein and mRNA of iNOS in LPS-activated microglial cells at 10 muM as observed in Western blot analysis and RT-PCR experiment. Furthermore they inhibited the degradation of I-kappaB-alpha in activated microglia. These results imply that compounds 1 and 2 can be lead compounds for the development of neuroprotective drug with the inhibitory activity of NO overproduction by activated microglial cells.
A case of acute cholestatic hepatitis associated with the seeds of Psoralea corylifolia (Boh-Gol-Zhee).:Clin Toxicol (Phila). 2005;43(6):589-91.Nam SW, Baek JT, Lee DS, Kang SB, Ahn BM, Chung KW.Department of Internal Medicine, Medical College of the Catholic University of Korea, Seoul, Korea.
The potential hepatotoxicity of herbal remedies is usually ignored in daily life. One such compound, Boh-Gol-Zhee (in Chinese, Bu Ku Zi), appeared to be associated with the occurrence of acute cholestatic hepatic injury in the following case. Some alternative medicine therapists claim that Psoralea corylifolia is effective for the treatment of osteoporosis. We observed a case of acute cholestatic hepatitis associated with the use of the seeds of Psoralea corylifolia in amounts over 10 times the usual dose in a postmenopausal woman. Liver biopsy showed zone three necroses, degenerating cells, cholestasis, and infiltrations with inflammatory cells. This case stresses the need to warn of the potential hepatotoxicity of the seed of Psoralea corylifolia, especially in a large dose.
Amplified fragment length polymorphism and metabolomic profiles of hairy roots of Psoralea corylifolia L.:Phytochemistry. 2005 Oct;66(20):2441-57. Epub 2005 Oct 5.Abhyankar G, Reddy VD, Giri CC, Rao KV,Lakshmi VV, Prabhakar S, Vairamani M, Thippeswamy BS, Bhattacharya PS.Centre for Plant Molecular Biology, Osmania University, Hyderabad, India.
A reproducible protocol for establishment of hairy root cultures of Psoralea corylifolia L. was developed using Agrobacterium rhizogenes strain ATCC 15834. The hairy root clones exhibited typical sigmoid growth curves. Genomic and metabolomic profiles of hairy root clones along with that of untransformed control were analysed. Hairy root clones, Ps I and Ps II, showed significant differences in their amplified fragment length polymorphism (AFLP) profiles as compared to that of control, besides exhibiting Ri T-DNA-specific bands. These results amply indicate the stable integration of Ri T-DNA into the genomes of these clones. Further, the variations observed between clones in the AFLP profiles suggest the variable lengths and independent nature of Ri T-DNA integrations into their genomes. An isoflavonoid, formononetin, and its glycoside were present only in the hairy root clones while they were absent in the untransformed control. Variations observed in the metabolite profiles of these clones may be attributed to the random T-DNA integrations and associated changes caused by them in the recipient genomes. GC/MS analyses revealed the production of three and six clone-specific compounds in Ps I and Ps II, respectively, suggesting that the clones are dissimilar in their secondary metabolism. HPLC/UV-MS analyses disclosed substantial increases in the total isoflavonoids produced in Ps I (184%) and Ps II (94%) compared to untransformed control.
Integration of ion-exchange chromatography fractionation with reversed-phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometer and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for isolation and identification of compounds in Psoralea corylifolia.:J Chromatogr A. 2005 Sep 30;1089(1-2):87-100.Chen X, Kong L, Su X, Pan C, Ye M, Zou H.National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
An approach for the separation and identification of components in a traditional Chinese medicine Psoralea corylifolia was developed. Ion-exchange chromatography (IEC) was applied for the fractionation of P. corylifolia extract, and then followed by concentration of all the fractions with rotary vacuum evaporator. Each of the enriched fractions was then further separated on an ODS column with detection of UV absorbance and atmospheric pressure chemical ionization mass spectrometer (APCI/MS), respectively, and also analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) with matrix of oxidized carbon nanotubes. Totally more than 188 components in P. corylifolia extract were detected with this integrated approach, and 12 of them were preliminary identified according to their UV spectra and mass spectra performed by APCI/MS and MALDI-TOF/MS. The obtained analytical results not only demonstrated the powerful resolution of integration IEC fractionation with reversed-phase liquid chromatography (RPLC)-APCI/MS and MALDI-TOF/MS for analysis of compounds in a complex sample, but also exhibited the superiority of APCI/MS and MALDI-TOF/MS for identification of low-mass compounds, such as for study of traditional Chinese medicines (TCMs) and metabolome.
A new biologically active flavonol glycoside from Psoralea corylifolia (Linn.).:J Asian Nat Prod Res. 2005 Aug;7(4):671-5.Yadava RN, Verma V.Department of Chemistry, Natural Products Laboratory, Dr. H. S. Gour University Sagar, Sagar, M.P. 470002, India. rnyadava@rediffmail.com
A new biologically active flavonol glycoside (1) mp 264-265 degrees C, C32H38O20, [M]+ 742 (EIMS) has been isolated from the methanol-soluble fraction of the defatted seeds of Psoralea corylifolia (Linn.). It was characterised as the new flavonol glycoside 3,5,3',4'-tetrahydroxy-7-methoxyflavone-3'-O-alpha-L-xylopyranosyl(1-->3)-O-alpha-L-arabinopyranosyl(1-->4)-O-beta-D-galactopyranoside by several colour reactions, spectral analysis and chemical degradations. Compound 1 showed anti-microbial activity against various bacteria and fungi.
Analysis of Psoralea corylifolia L. fruits in different regions.:Chem Pharm Bull. 2005 Aug;53(8):1054-7.Zhao LH, Wu MH, Xiang BR.Analytic Center, China Pharmaceutical University, Nanjing 210009, China. zhaoLuhua@hotmail.com
Application of multivariate data analysis has become a popular method in the last decades, mainly because it can provide information not otherwise accessible. The information includes classification, searching similarities, finding relationships, finding physical significance to principal components, etc. Twenty-two Chinese medicinal herbs containing twelve constituents were collected and determined by HPLC. The results were studied by hierarchical cluster analysis (HCA) and principal components analysis (PCA). It was shown that the samples could be clustered reasonably into three groups, hence corresponding with the typical habitats of Psoralea corylifolia L.
Protective effect of (S)-bakuchiol from Psoralea corylifolia on rat liver injury in vitro and in vivo.:Planta Med. 2005 Jun;71(6):508-13.
The aim of this study was to investigate the protective effect of (S)-bakuchiol isolated from the seed of Psoralea corylifolia, on liver injury. Primary rat hepatocyte intoxication was induced by tert-butyl hydroperoxide (tBH), carbon tetrachloride (CCl4) or D-galactosamine (D-GalN). Liver injury was induced by either CCl4 or D-GalN in rats. In vitro, the cellular leakage of lactate dehydrogenase and cell viability following treatment with hepatotoxicants were significantly improved by bakuchiol treatment at a concentration range of 25-200 microM for tBH, 100-200 microM for CCl4 and 100-200 microM for D-GalN-induced hepatocyte injury. Treatment with bakuchiol significantly inhibited lipid peroxidation and intracellular glutathione depletion in hepatocytes induced by tBH, CCl4 or D-GalN. Treatment with bakuchiol (25 or 50 mg/kg, p.o.) at 1, 24 and 48 h after subcutaneous injection of CCl4 significantly reduced the levels of aspartate transaminase and alanine transaminase in serum. Histological observations revealed that fatty acid changes, hepatocyte necrosis and inflammatory cell infiltration in CCl4-injured liver was improved when treated with bakuchiol. Bakuchiol treatment (25 and 50 mg/kg, p.o.) also significantly reduced the levels of aspartate transaminase and alanine transaminase in an acute liver injury model induced by D-GalN. From these results, bakuchiol has a protective effect against tBH, CCl4 or D-GalN-induced hepatotoxicity in vitro or in vivo.
Extraction and purification of psoralen from Psoralea corylifolia L.:Di Yi Jun Yi Da Xue Xue Bao. 2005 Jun;25(6):751-2. Chinese.Liu ZL, Zhang XN, Zhao SJ, Zhou RX.Outpatient Clinic, Guangdong University of Foreign Studies, Guangzhou 510420, China.
OBJECTIVE: To establish a method for extracting psoralen from the seed of Psoralea corylifolia L. METHODS: Crushed seed of Psoralea corylifolia L. was soaked with 50% ethyl alcohol solution, and the leaching solution was filtrated and distilled to remove alcohol. A slurry of the sediment was obtained and dissolved in methyl alcohol (MeOH), followed by treatment with active carbon for decoloration and evaporation of MeOH, resulting in a lamellar crystal after the sediment was kept still overnight. MeOH was applied to dilute the sticky solution, and after filteration, a yellow crystal was obtained, recrystallization of which in MeOH yielded white needle-like crystals. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were employed to determine the purity of the resultant substance, and UV and H1-NMR applied to identify its chemical structure. RESULTS AND CONCLUSION: The resultant crystal is confirmed to be pure monomers of psoraren with a yield of 0.147% from the seed (m/m) by this simple extraction method.
Fingerprint analysis of Psoralea corylifolia L. by HPLC and LC-MS.:J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jul 5;821(1):67-74.Zhao L, Huang C, Shan Z, Xiang B, Mei L.Analytic Center, China Pharmaceutical University, No. 24 Tongjiaxiang Road, Nanjing, Jiangsu 210009, China. zhaoluhua@hotmail.com
High-performance liquid chromatography (HPLC) was developed for fingerprint analysis of Psoralea corylifolia. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MSn) technique was first employed to identify the components of the fingerprint. The samples were separated with an Alltima C18 column (250 mm x 4.6 mm, 5 microm) by linear gradient elution using water-acetic acid (A; 100:0.1, v/v) and acetonitrile (B; 0 min, 40%; 15 min, 50%; 35 min, 60%; 45 min, 70%; 55 min, 80%; and maintained for 5 min) as mobile phase at a flow rate of 1.0 ml/min and detector wavelength at 245 nm. A standard procedure was developed for HPLC fingerprint analysis. Average chromatogram of 10 batches of P. corylifolia L. from Sichuan and Henan Provinces, PR China, which has been considered as the original and genuine herbal medicine for a long time, was first established as the characteristic fingerprint. There are 12 common peaks in this fingerprint. Ten of these common peaks were identified by MS data. This profile was then used to identify and assess the differences among the herb grown in various areas of China. The HPLC fingerprint analysis is specific and may serve for quality identification and comprehensive evaluation of P. corylifolia.
In vitro estrogenic activities of Chinese medicinal plants traditionally used for the management of menopausal symptoms:J Ethnopharmacol. 2005 Apr 26;98(3):295-300.Zhang CZ, Wang SX, Zhang Y, Chen JP, Liang XM.Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road No. 161, Dalian 116011, PR China.
The estrogenic activity of 70% EtOH extracts of 32 traditional Chinese medicinal plants, selected according to their reported efficacy for the treatment of menopausal symptoms, was assessed using a recombinant yeast system with both a human estrogen receptor expression plasmid and a reporter plasmid. Among them, 11 (34%) species proved to be active. Polygonum cuspidatum had the highest estrogenic relative potency (RP) (3.28 x 10(-3)), followed by Rheumpalmatum (3.85 x 10(-4)), Cassia obtusifolia (3.49 x 10(-4)), Polygonum multiflorum (2.87 x 10(-4)), Epimedium brevicornum (2.30 x 10(-4)), Psoralea corylifolia (1.90 x 10(-4)), Cynomorium songaricum (1.78 x 10(-4)), Belamcanda chinensis (1.26 x 10(-4)), Scutellaria baicalensis (8.77 x 10(-5)), Astragalus membranaceus (8.47 x 10(-5)) and Pueraria lobata (6.17 x 10(-5)). The EC(50) value of 17beta-estradiol used as the positive control was 0.205+/-0.025 ng/ml (RP=100). This study gave support to the reported efficacy of Chinese medicines used for hormone replacement therapy.
Determination of psoralen and isopsoralen in Psoralea corylifolia L. from different habitats.:Zhong Yao Cai. 2004 Nov;27(11):817-8. Chinese.Ding J, Zhang Q, Zhang L.Institute of Chinese Medicine Basic Theory, China Academy of Traditional Chinese Medicine, Beijing.
OBJECTIVE: To study the psoralen and isopsoralen content in Psoralea corylifolia from different habitats. METHOD: C18 column was used. Mobile phase was methanol-water (45:55). The detection wavelength was at 245nm and the flow rate was 1.0 ml/min. RESULT: The linearity was obtained over the range of 0.448 to approximately 0.448 microg (r = 0.9999) for psoralen, 0.356 to approximately 0.356 microg = 0.997) for isopsoralen. CONCLUSION: The contents of psoralen and isopsoralen in Psoralea corylifolia from different habitats have no obvious difference.
In vitro protein tyrosine phosphatase 1B inhibitory phenols from the seeds of Psoralea corylifolia.:Planta Med. 2005 Jan;71(1):87-9.Kim YC, Oh H, Kim BS, Kang TH, Ko EK, Han YM, Kim BY, Ahn JS.Phytofermentation Research Center and College of Pharmacy, Wonkwang University, Iksan, South Korea.
Protein tyrosine phosphatase 1B plays a major role in the negative regulation of insulin signaling, and this establishes protein tyrosine phosphatase 1B as an attractive therapeutic target for diabetes. Bioassay-guided fractionation of the EtOAc-soluble extract of the seeds of Psoralea corylifolia afforded two protein tyrosine phosphatase 1B inhibitory compounds, psoralidin (1) and bakuchiol (2), along with inactive corylin. Compounds 1 and 2 inhibited PTP1B activity in a dose-dependent manner, displaying IC50 values of 9.4 +/- 0.5 microM and 20.8 +/- 1.9 microM, respectively.
Behavioral and biochemical studies of total furocoumarins from seeds of Psoralea corylifolia in the forced swimming test in mice.:J Ethnopharmacol. 2005 Jan 15;96(3):451-9. Epub 2004 Nov 19.Liu ZL, Zhang XN, Zhao SJ, Zhou RX.Outpatient Clinic, Guangdong University of Foreign Studies, Guangzhou 510420, China.
OBJECTIVE: To establish a method for extracting psoralen from the seed of Psoralea corylifolia L. METHODS: Crushed seed of Psoralea corylifolia L. was soaked with 50% ethyl alcohol solution, and the leaching solution was filtrated and distilled to remove alcohol. A slurry of the sediment was obtained and dissolved in methyl alcohol (MeOH), followed by treatment with active carbon for decoloration and evaporation of MeOH, resulting in a lamellar crystal after the sediment was kept still overnight. MeOH was applied to dilute the sticky solution, and after filteration, a yellow crystal was obtained, recrystallization of which in MeOH yielded white needle-like crystals. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were employed to determine the purity of the resultant substance, and UV and H1-NMR applied to identify its chemical structure. RESULTS AND CONCLUSION: The resultant crystal is confirmed to be pure monomers of psoraren with a yield of 0.147% from the seed (m/m) by this simple extraction method.
Preparation and in vitro evaluation of radioiodinated bakuchiol as an anti tumor agent.:Appl Radiat Isot. 2005 Mar;62(3):389-93.Bapat K, Chintalwar GJ, Pandey U, Thakur VS, Sarma HD, Samuel G, Pillai MR, Chattopadhyay S, Venkatesh M.Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
Bakuchiol, extracted from the plant Psoralea corylifolia, has been proven to have anti-tumor, cytotoxic, anti-microbial and anti-inflammatory activity. In order to study if radiolabeled bakuchiol exhibits enhanced cytotoxicity, bakuchiol was radiolabeled with 125I. In-vitro uptake studies of 125I-bakuchiol were carried out using LS-A (lymphosarcoma) and barcl-95 (radiation-induced thymic lymphoma) ascitic and solid tumor cells of murine origin. In both LS-A and barcl-95, 125I-bakuchiol showed significant uptake. Viability studies showed that the radioiodinated compound showed greater cytotoxic effect than bakuchiol.
Preparative isolation and purification of psoralen and isopsoralen from Psoralea corylifolia by high-speed counter-current chromatography.:J Chromatogr A. 2004 Nov 19;1057(1-2):225-8.Liu R, Li A, Sun A, Kong L.
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China. renminliu@lctu.edu.cn
Psoralen and isopsoralen were separated from Psoralea corylifolia by high-speed counter-current chromatography (HSCCC). A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:5:4.5:5.5, v/v) was used for HSCCC separation, and yielded, from 100 mg of crude extract, 39.6 mg of psoralen and 50.8 mg of isopsoralen each at over 99% purity as determined by high performance liquid chromatography (HPLC). The identification of psoralen and isopsoralen were performed with 1H NMR and 13C NMR.
Antibacterial prenylflavone derivatives from Psoralea corylifolia, and their structure-activity relationship study.:Bioorg Med Chem. 2004 Aug 15;12(16):4387-92.Yin S, Fan CQ, Wang Y, Dong L, Yue JM.State Key Laboratory of Drug Research, Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, PR China.
Three new prenylflavonoids, namely corylifols A-C (1-3), together with 13 known ones, were isolated from the seeds of Psoralea corylifolia. Their structures were elucidated by spectral methods including 1D and 2D NMR techniques. All the isolates were tested on antibacterial assays, and nine of them showed significant antibacterial activities against two pathogenic bacteria Staphylococcus aureus and S. epidermidis. The antibacterial structure-activity relationship of these prenylflavonoids (1-16) was also briefly discussed.
Characterization of antioxidant and antiglycation properties and isolation of active ingredients from traditional chinese medicines.:Free Radic Biol Med. 2004 Jun 15;36(12):1575-87.Tang SY, Whiteman M, Peng ZF, Jenner A, Yong EL, Halliwell B.Department of Biochemistry, Faculty of Medicine, National University of Singapore, Singapore.
There is considerable interest in the isolation of more potent antioxidant compounds to treat diseases involving oxidative stress. Thirty-three traditional Chinese medicine (TCM) extracts were examined for their antioxidant activity using the 2,2'-azinobis[3-ethylbenzothiazoline-6-sulfonate] (ABTS) assay. Five extracts with high activity (Cratoxylum cochinchinense, Cortex magnoliae officinalis, Psoralea corylifolia L, Curculigo orchioides Gaertn, and Glycyrrhiza uralensis Fisch.) were selected for further characterization. C. cochinchinense outperformed other extracts in most of the assays tested except phospholipid peroxidation inhibition, where P. corylifolia L showed higher activity. C. cochinchinense was particularly potent in inhibiting the formation of advanced glycation end products on proteins and strongly inhibited hypochlorous acid-induced DNA damage. We attempted to isolate the active ingredients from C. cochinchinense and obtained an extract (YCT) containing at least 90% mangiferin as identified by HPLC and mass spectrometry. However, YCT showed significantly higher activity in assays of phospholipid peroxidation, inhibition of protein glycation, and superoxide (O(2)(?-)) and peroxynitrite (ONOO(-)) scavenging, as compared with mangiferin, suggesting that the nonmangiferin constituents of YCT contribute to its additional antioxidant activities.
Antidermatophytic activity of extracts from Psoralea corylifolia (Fabaceae) correlated with the presence of a flavonoid compound.:J Ethnopharmacol. 2004 Mar;91(1):21-4.Rajendra Prasad N, Anandi C, Balasubramanian S, Pugalendi KV.Department of Biochemistry, Annamalai University, Annamalainagar 608 002, Tamilnadu, India.
Extracts obtained from seeds of Psoralea corylifolia showed several degrees of antifungal activity against Trichophyton rubrum, Trichophyton mentagrophytes, Epidermophyton floccosum and Microsporum gypseum by the disc diffusion method on a Sabouraud dextrose agar (SDA) medium. Methanol extract of the seeds at 250 microg exhibited the maximum inhibition with a halo of 28 mm diameter. Six different bands were obtained when the methanol extract was subjected to TLC. 13C NMR and Mass spectra revealed that the active compound would be a flavonoid, 4'-methoxy flavone. MIC of the active compound along with standard miconazole was carried out using tube dilution technique.
Antibacterial compounds from the seeds of Psoralea corylifolia.:Fitoterapia. 2004 Mar;75(2):228-30.Khatune NA, Islam ME, Haque ME, Khondkar P, Rahman MM.Phytochemistry Research Laboratory, Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh.
Psoralidin, bakuchicin, psoralin and angelicin, isolated from the seeds of Psoralea corylifolia, showed significant antibacterial activities against a number of Gram (+) and Gram (-) bacteria.
Osteoblastic differentiation bioassay and its application to investigating the activity of fractions and compounds from Psoralea corylifolia L.:Pharmazie. 2003 Dec;58(12):925-8.Xiong Z, Wang D, Xu Y, Li F.School of Pharmacy, Shenyang Pharmaceutical University, China.
A cell differentiation bioassay measuring alkaline phosphatase activity was developed using osteoblast-like UMR 106 cell line as a model. The effect of fractions and compounds of Psoralea corylifolia L. extract on osteoblastic differentiation was investigated. The fractions or compounds were co-cultured with cells for 48 h, the cellular ALP activity was then measured. The crude ethanol extract of Psoralea corylifolia L. increased ALP activity by 39.5% at a concentration of 0.1 mg/ml, and the activity was concentrated in the ethyl acetate fraction, which produced a maximum ALP activity increase of 38.2%. A significant activity was observed for a flavonoid, corylin, providing 30.0% increment in ALP. These effects suggest that Psoralea corylifolia L. extract, its ethyl acetate fraction and corylin would stimulate bone formation.
Sequential analysis of testicular lesions and serum hormone levels in rats treated with a Psoralea corylifolia extract.:Food Chem Toxicol. 2004 Jan;42(1):1-7.
In order to clarify pathogenetic targets for the testicular toxicity of a extract of Psoralea corylifolia (P. corylifolia), F344 rats were fed diet containing 3% P. corylifolia extract for up to 12 weeks and subjected to hormone assays and histopathological examination on the testis and epididymis at weeks 1, 2, 4, 8 and 12 (Exp 1). Similar analyses were performed on 1, 3, 7 and 14 days after a single gavage administration of the P. corylifolia extract at a dose of 10 g/kg b.w. (Exp 2). In Exp 1, increase in the numbers of degenerated and exfoliated germ cells and loss of elongated spermatids beyond steps 7 or 8 were initially observed in the seminiferous tubules at week 1, followed by more pronounced degeneration of germ cells with depletion of post-meiotic populations from week 2. The tubular degeneration was associated with Leydig cell atrophy and persistent reduction of serum testosterone and FSH levels throughout the treatment period and a slight reduction of serum LH in later stages. In Exp 2, reduction of serum testosterone and FSH levels preceded degeneration of germ cells in stage VII and VIII tubules at 3 and 7 days after the administration. The results suggest that rapid androgen deprivation reflecting direct interference with Leydig cell function and simultaneous disturbance of the pituitary-testicular axis play pivotal roles in P. corylifolia extract-induced germ cell injury in seminiferous tubules
Quantitative determination of psoralen and angelicin from some medicinal herbs by high performance liquid chromatography.:Arch Pharm Res. 2003 Jul;26(7):516-20.
A reversed-phase high performance liquid chromatographic method was developed to determine the contents of psoralen and angelicin from some medicinal herbs. The optimum eluent for chromatography was 20 v/v% acetonitrile in water on a Zorbax 300SB C18 column. The identification was carried out by comparing the retention time and mass spectra of the relevant peaks with their standards. The variation of the concentration of psoralen and angelicin was wide between different species. The seeds of Psoralea corylifolia showed the highest contents of psoralen (7.8 mg/g) and angelicin (2.3 mg/g) among the tested herbs.
Studies on extraction and isolation of active constituents from Psoralen corylifolia L. and the antitumor effect of the constituents in vitro.:Zhong Yao Cai. 2003 Mar;26(3):185-7. Chinese.Guo J, Wu H, Weng X, Yan J, Bi K.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016.
OBJECTIVE: To study the chemical constituents from Psoralea corylifolia L. and their antitumor activities. METHODS: The constituents were isolated on silica gel column chromatography and identified by physicochemical properties and spectroscopic analysis. Morphological and MTT assays in vitro were used to explore the inhibiting effect of the active compounds on the proliferation of BGC-823 cancer cell. RESULTS: Two compounds were isolated from its chloroform extract and identified as psoralen (I) and isopsoralen (II). IC50 of psoralen and isoporalen were 5.82 micrograms/ml and 148.8 micrograms/ml respectively. CONCLUSION: Compound I and II having antitumor activity against BGC-823 cancer cell were obtained from this plant with the yields of 0.048% and 0.11%.
HPLC determination of two flavonoid compounds in Psoralea corylifolia.:Zhongguo Zhong Yao Za Zhi. 2002 Sep;27(9):669-71. Chinese.Lin RM, Wang DW, Xiong ZL, Xu Y, Li FM.Department of Chemistry, Anshan Teacher's College, Anshan 114005, Liaoning, China.
OBJECTIVE: To determine two flavonoid compounds in Psoralea corylifolia L. (PC) simultaneously with HPLC method. METHOD: Bavachin and corylin isolated from PC and purified in our laboratory were used as the reference compounds. The HPLC separation was carried out on an Techsphere ODS column using mobile phase consisting of a mixture of methanol and 20 mmol.L-1 ammonium acetate buffer pH 4.0 (67:33), and the UV detection wavelength was 240 nm. RESULT: Simultaneous determination of bavachin and corylin was achieved. The linear range was 1.25-20 micrograms.mL-1 for both bavachin and corylin. The average recovery of bavachin and corylin was 94.9% and 96.2%, and RSD was 3.1% and 3.6% respectively. CONCLUSION: This is the first report on simultaneous determination of bavachin and corylin in PC with satisfactory accuracy and reproducibility.
Ethanolic extracts of Euphorbia and other ethnobotanical species as inhibitors of human tumour cell growth.:Phytomedicine. 2003 Jan;10(1):53-8.Whelan LC, Ryan MF.Department of Zoology, University College Dublin, Belfield, Dublin, Ireland.
Ethanolic extracts of 20 plant species, selected from the ethnobotanical literature, were analysed for their pharmacological potential as antineoplastic agents against the HEp-2 cell line. Psoralea corylifolia and E. grandidens were the most efficacious species eliciting IC50 values of 22 microg/ml and 57 microg/ml respectively. Psoralea corylifolia, additionally tested against lung carcinoma (A549) cells gave an IC50 value of 68 microg/ml. Such data would justify a search for active compounds from this species.
Determination of major components in Psoralea corylifolia L. by using supercritical fluid extraction (SFE).:Se Pu. 2000 Jan;18(1):61-3. Chinese.Chen B, Liu LL, Zhai ZX, Chen W, Fang HS, Wu YT.School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
A method based on SFE and CGC has been developed for qualitative and quantitative analysis of major components in Psoralea corylifolia L.. The orthogonal design method and the analysis of variance are used to optimize the five variables (temperature, pressure, volume of modifier, static extraction time and CO2 dynamic extracting volume) of SFE and the optimized conditions listed as follows: temperature 70 degrees C, pressure 38.5 MPa, static extraction 1 min, dynamic extraction 7 mL and 60 microL CHCl3 as modifier. The recoveries of psoralen and isopsoralen are 96.93% (RSD 1.87%) and 98.43% (RSD 2.06%) respectively. Being compared with conventional methods, the SFE as an alternative technique for sample preparation will be most useful in the quality control of Chinese traditional medicines.
Antioxidative components of Psoralea corylifolia (Leguminosae).:Phytother Res. 2002 Sep;16(6):539-44.
A meroterpene and four flavonoids were isolated from the seeds of Psoralea corylifolia as antioxidative components. Their structures were elucidated by spectral data and identified as bakuchiol (1), bavachinin (2), bavachin (3), isobavachin (4) and isobavachalcone (5). In particular, meroterpene 1 and flavonoids 4 and 5 showed broad antioxidative activities in rat liver microsomes and mitochondria. They inhibited NADPH-, ascorbate-, t-BuOOH- and CCl(4)-induced lipid peroxidation in microsomes. They also prevented NADH-dependent and ascorbate-induced mitochondrial lipid peroxidation. Bakuchiol (1) was the most potent antioxidant in microsomes and the inhibition of oxygen consumption induced by lipid peroxidation was time-dependent. Furthermore, bakuchiol (1) protected human red blood cells against oxidative haemolysis. These phenolic compounds in P. corylifolia were shown to be effective in protecting biological membranes against various oxidative stresses.
A study on nasal absorption of psoralen and isopsoralen in Psoralea corylifolia L.:Zhongguo Zhong Yao Za Zhi. 1999 Nov;24(11):689-91, 704. Chinese.Li A, Wang H.Central Laboratory of Guangdong Provincial Hospital of TCM, Guangzhou 510120.
OBJECTIVE: To investigate the nasal absorption regularities of psoralen and isopsoralen of different concentrations. METHOD: Building an experimental model of rat in situ nasal recirculation and determining the contents of psoralen and isopsoralen by HPLC. RESULT: The nasal absorption of psoralen and isopsoralen fitted in with zero order kinetics, getting saturated with the increase of concentration. CONCLUSION: A suitable concentration is necessary for the preparation of nasal remedies psoralen and isopsoralen.
Potential antifilarial activity of the leaves and seeds extracts of Psoralea corylifolia on cattle filarial parasite Setaria cervi.:J Ethnopharmacol. 2002 Sep;82(1):23-8. Erratum in: J Ethnopharmacol. 2003 Feb;84(2-3):313.Qamaruddin , Parveen N, Khan NU, Singhal KC.Department of Chemistry, Aligarh Muslim University, Aligharh 202 002, India. quamaruahmed@yahoo.com
The effect of aqueous and alcohol extracts of the leaves and seeds of Psoralea corylifolia, on the spontaneous movements of both the whole worm and the nerve muscle preparation of Setaria cervi and on the survival of microfilariae in vitro was studied. Alcohol extracts of both leaves and seeds caused the inhibition of spontaneous movements of the whole worm and the nerve muscle preparation of S. cervi, characterised by initial, short lasting small increase in tone of contractions followed by paralysis. The initial stimulatory effect was not observed by alcohol extract of leaves on nerve muscle preparation. The concentrations required to inhibit the movements of whole worm and nerve muscle preparations for alcohol extracts of leaves and seeds were 160, 30, and 150, 20 microg/ml, respectively suggesting a cuticular permeability barrier. Alcohol extracts of both leaves and seeds caused death of microfilariae in vitro, LC(50) and LC(90) being 15 and 25 ng/ml for alcohol extract of leaves and 12 and 18 ng/ml for alcohol extract of seeds, respectively.
Gonadal toxicity of an ethanol extract of Psoralea corylifolia in a rat 90-day repeated dose study.:J Toxicol Sci. 2002 May;27(2):97-105.
Ethanol extracts of seeds of Psoralea corylifolia are proposed as food additives for processed food preservation. An extract was administered by admixing into diet at concentrations of 0, 0.375, 0.75, 1.5 or 3.0% to 10 male and 10 female F344 rats each for 90 days to evaluate its toxicity. Body weight gain, food consumption and food conversion efficiency (body weight gain per food consumption) were lower in the extract-treated animals, except for the 0.375% males, as compared to the control animals. Absolute and/or relative testes weights in the 1.5 and 3.0% groups and those of ovaries in the 3.0% group were significantly (p < 0.01) lower than in the control group. On histopathological examination, seminiferous tubular atrophy and Leydig cell atrophy in the testes, and epithelial cell atrophy in the seminal vesicles and prostate were observed in the 1.5 and 3.0% males. Decrease in the number of corpora lutea associated with frequent necrotic follicles in the ovaries in the 1.5 and 3.0% females and less frequent endometrial glands in the uterus in the 3.0% females were also detected. These results might suggest disruption of the hypothalamus-pituitary-gonadal axis in Psoralea corylifolia-treated rats as possible mechanisms underlying this gonadal toxicity.
Effects of herbal preparation Equiguard on hormone-responsive and hormone-refractory prostate carcinoma cells: mechanistic studies.:Int J Oncol. 2002 Apr;20(4):681-9.Hsieh TC, Lu X, Guo J, Xiong W, Kunicki J, Darzynkiewicz Z, Wu JM.Department of Biochemistry and Molecular Biology, Brander Cancer Research Institute, New York Medical College, Valhalla, NY 10595, USA.
The Equiguard is a dietary supplement comprised of standardized extracts from nine herbs, respectively, Herba epimedium brevicornum Maxim (stem and leaves), Radix morindae officinalis (root), Fructus rosa laevigatae michx (fruit), Rubus chingii Hu (fruit), Schisandra chinensis (Turz.) Baill (fruit), Ligustrum lucidum Ait (fruit), Cuscuta chinensis Lam (seed), Psoralea corylifolia L. (fruit), and Astragalus membranaceus (Fisch.) Bge (root). This proprietary product, formulated according to Chinese traditional medicinal concepts, is aimed at restoring harmony in the of the kidney, an organ which Chinese medicinal principles consider to be vital for invigorating as well as maintaining balance of the entire urological system. As the prostate is an integral component of the urological system, we performed in vitro studies to test the effects of ethanol extracts of Equiguard to modulate prostate growth and gene expression. These studies used prostate cancer cells mimicking the androgen-dependent (AD) and androgen-independent (AI) states of prostate carcinogenesis. Results show that Equiguard significantly reduced cancer cell growth, induced apoptosis, suppressed expression of the androgen receptor (AR) and lowered intracellular and secreted prostate specific antigen (PSA), and almost completely abolished colony forming abilities of prostate cancer cells. These data support the interpretation that this herbal formulation contains ingredients that collectively may be efficacious in preventing or treating AD and AI prostate carcinoma. The anti-prostatic activities of Equiguard may stem from its complex composition capable of targeting multiple signal transduction/metabolic pathways, to effectively correct, counteract or circumvent the impaired or dysfunctional mechanisms accompanying different stages of prostate carcinogenesis.
The evaluation of forty-three plant species for in vitro antimycobacterial activities; isolation of active constituents from Psoralea corylifolia and Sanguinaria canadensis.:J Ethnopharmacol. 2002 Jan;79(1):57-67. Review.Newton SM, Lau C, Gurcha SS, Besra GS, Wright CW.The School of Pharmacy, University of Bradford, BD7 1DP, West Yorkshire, UK.
Extracts from forty-three plant species were selected on account of reported traditional uses for the treatment of TB and/or leprosy. These were assayed for antimycobacterial activities. A simple in vitro screening assay was employed using two model species of mycobacteria, M. aurum and M. smegmatis. Crude methanolic extracts from three of the plants, C. mukul, P. corylifolia and S. canadensis, were found to have significant antimycobacterial activity against M. aurum only (MIC=62.5 microg/ml). Bioassay guided fractionation led to the isolation of two known benzophenanthridine alkaloids, sanguinarine (1) and chelerythrine (2), from the roots S. canadensis and the known phenolic meroterpene, bakuchiol (3) from the seeds of P. corylifolia. The fractionation of the resin of C. mukul lead to a decrease in antimycobacterial activity and hence further work was not pursued. Compound (2) was the most active against M. aurum and M. smegmatis (IC(50)=7.30 microg/ml [19.02 microM] and 29.0 microg/ml [75.56 microM], respectively). M. aurum was the most susceptible organism to all three compounds. No significant difference in antimycobacterial activity was observed when the two alkaloids were tested for activity in media of differing pH values. The activities of the pure compounds against M. aurum were comparable with those against M. bovis BCG with compound (2) being the most active (M. bovis BCG, IC(50)=14.3 microg/ml [37.3 microM]). These results support the use of these plants in traditional medicine.
Bakuchiol: a hepatoprotective compound of Psoralea corylifolia on tacrine-induced cytotoxicity in Hep G2 cells.:Planta Med. 2001 Nov;67(8):750-1.Li A, Wang H.Central Laboratory of Guangdong Provincial Hospital of TCM, Guangzhou 510120.
OBJECTIVE: To investigate the nasal absorption regularities of psoralen and isopsoralen of different concentrations. METHOD: Building an experimental model of rat in situ nasal recirculation and determining the contents of psoralen and isopsoralen by HPLC. RESULT: The nasal absorption of psoralen and isopsoralen fitted in with zero order kinetics, getting saturated with the increase of concentration. CONCLUSION: A suitable concentration is necessary for the preparation of nasal remedies psoralen and isopsoralen.
Osteoblastic proliferation stimulating activity of Psoralea corylifolia extracts and two of its flavonoids.:Planta Med. 2001 Nov;67(8):748-9.Wang D, Li F, Jiang Z.Department of Analytical Chemistry, Shenyang Pharmaceutical University, China.
Psoralea corylifolia L. fruit extracts exhibited osteoblastic proliferation stimulating activity in UMR106 cell line cultured in vitro. The flavonoids of corylin and bavachin were isolated and identified as active principles by activity-guided fractionation. The results suggested that Psoralea corylifolia L. fruit extracts and corylin and bavachin might stimulate bone formation or have potential activity against osteoporosis.
The presence of three isoflavonoid compounds in Psoralea corylifolia.:J Chromatogr Sci. 2001 Oct;39(10):441-4.Hsu YT, Wu CJ, Chen JM, Yang YC, Wang SY.Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan.
The optimization of a high-performance liquid chromatographic method to determine three isoflavonoids (daidzein, genistein, and biochanin A) in the fruit of Psoralea corylifolia is developed and validated. Dried psoralea fruit powder is extracted with aqueous methanol followed by the hydrolysis of the analytes' conjugated glycosides with hydrochloric acid. The HPLC assay is performed on a reverse-phase C18 column with gradient elution using acetonitrile and 10% acetic acid as the mobile phase at a flow rate of 0.8 mL/min. Flavone is used as the internal standard and the substances are detected at 260 nm. Calibrations are linear (correlation coefficient > or = 0.995) for all three analytes. The limits of detection are 0.01 microg/mL for daidzein and genistein and 0.1 microg/mL for biochanin A. The overall intra- and interassay precision range from 2.5% to 4.9% and from 0.5% to 4.7%, respectively. The method proved to be sensitive, specific, accurate, and precise for the determination of daidzein, genistein, and biochanin A in Psoralea corylifolia.
In vitro antimicrobial activities of bakuchiol against oral microorganisms.:Antimicrob Agents Chemother. 2001 Nov;45(11):3009-13.
Bakuchiol was isolated from the seeds of Psoralea corylifolia, a tree native to China with various uses in traditional medicine, followed by extraction with ether and column chromatography combined with silica gel and octyldecyl silane. In this study, the antimicrobial activities of bakuchiol against some oral microorganisms were evaluated in vitro. The cell growth of Streptococcus mutans was inhibited in a bakuchiol concentration-dependent manner, and growth of S. mutans was completely prevented by 20 microg of bakuchiol per ml. The bactericidal effect of bakuchiol on S. mutans was dependent on temperature and stable under the following conditions: sucrose, 0 to 10% (wt/vol); pH, 3.0 to 7.0; organic acids (3% [wt/vol] citric and malic acids). Bakuchiol showed bactericidal effects against all bacteria tested, including S. mutans, Streptococcus sanguis, Streptococcus salivarius, Streptococcus sobrinus, Enterococcus faecalis, Enterococcus faecium, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Actinomyces viscosus, and Porphyromonas gingivalis, with MICs ranging from 1 to 4 microg/ml and the sterilizing concentration for 15 min ranging from 5 to 20 microg/ml. Furthermore, bakuchiol was also effective against adherent cells of S. mutans in water-insoluble glucan in the presence of sucrose and inhibited the reduction of pH in the broth. Thus, bakuchiol would be a useful compound for development of antibacterial agents against oral pathogens and has great potential for use in food additives and mouthwash for preventing and treating dental caries.
Cytotoxic constituents of Psoralea corylifolia.:Arch Pharm Res. 2001 Jun;24(3):211-3.
A coumestan derivative, psoralidin (1) was found to be a cytotoxic principle of the seeds of Psoralea corylifolia L. (Leguminosae) with the IC50 values of 0.3 and 0.4 microg/ml against the HT-29 (colon) and MCF-7 (breast) human cancer cell lines, respectively. A coumarin, angelicin (2) was also isolated as a marginally cytotoxic agent along with an inactive compound, psoralen (3) from the plant. The isolates 1-3 were not active against the A541 (lung) and HepG2 (liver hepatoma) cancer cell lines.
Selective isolation of isopsoralen from crude extract of Psoralea corylifolia. L by using inclusion method of host-guest molecules.:Yao Xue Xue Bao. 2000 Nov;35(11):864-7. Chinese.Guo WS, Guo F, Xu ZX, Zheng QT, Lu Y.Institute of Chemical Science and Engineering, Liaoning, University, Shenyang 110036, China. wensguo@lnu.edu.cn
AIM: To isolate the chemical components from extracted crude of Psoralea corylifolia L. METHODS: Applicate the function of molecular recognition in supramolecular chemistry, use 1,1,6,6-tetraphenylhexa-2,4-diyne-1,6-diol as the host molecule and chemical components in extracted crude of Psoralea corylifolia L. as guest molecules, which represent different from either in sort, quantity and sites of function groups or in structural topological character, 1,1,6,6-tetraphenylhexa-2,4-diyne-1,6-diol could selectively form inclusion compound with component endowed with interactional complementarity and isolate as crystalline from the extracted crude of Psoralea corylifolia L. RESULTS: The isopsoralen as guest molecule is selectively included by 1,1,6,6-tetraphenylhexa-2,4-diyne-1,6-diol in inclusion crystal and removed by acetone from the inclusion compound and total yield is 0.18%. Isopsoralen is determined by UV, IR, 1HNMR and MS and its inclusion compound is determined by means of IR, powder XRD and single crystal XRD. The isolation effect is analyzed by GC/MS. CONCLUSION: The method is simple and selective for isolation isopsoralen from extracted crude of Psoralea corylifolia L.
Inhibition of rat brain monoamine oxidase activities by psoralen and isopsoralen: implications for the treatment of affective disorders.:Pharmacol Toxicol. 2001 Feb;88(2):75-80. Kong LD, Tan RX, Woo AY, Cheng CH.State Key Laboratory of Pharmaceutical Biotechnology, Institute of Pharmaceutical Biology and Phytochemistry, Nanjing University, NT, Hong Kong, China.
Psoralen and isopsoralen, furocoumarins isolated from the plant Psoralea corylifolia L., were demonstrated to exhibit in vitro inhibitory actions on monoamine oxidase (MAO) activities in rat brain mitochondria, preferentially inhibiting MAO-A activity over MAO-B activity. This inhibition of enzyme activities was found to be dose-dependent and reversible. For MAO-A, the IC50 values are 15.2 +/- 1.3 microM psoralen and 9.0 +/- 0.6 microM isopsoralen. For MAO-B, the IC50 values are 61.8 +/- 4.3 microM psoralen and 12.8 +/- 0.5 microM isopsoralen. Lineweaver-Burk transformation of the inhibition data indicates that inhibition by both psoralen and isopsoralen is non-competitive for MAO-A. The Ki values were calculated to be 14.0 microM for psoralen and 6.5 microM for isopsoralen. On the other hand, inhibition by both psoralen and isopsoralen is competitive for MAO-B. The Ki values were calculated to be 58.1 microM for psoralen and 10.8 microM for isopsoralen. These inhibitory actions of psoralen and isopsoralen on rat brain mitochondrial MAO activities are discussed in relation to their toxicities and their potential applications to treat affective disorders.
Inhibition of mitochondrial lipid peroxidation by Bakuchiol, a meroterpene from Psoralea corylifolia.:Planta Med. 2000 Aug;66(6):569-71.
Bakuchiol, a meroterpene isolated from Psoralea corylifolia, prevented mitochondrial lipid peroxidation. Inhibition of oxygen consumption originating in lipid peroxidation was time-dependent. Bakuchiol protected mitochondrial respiratory enzyme activities against both NADPH-dependent and dihydroxyfumarate-induced peroxidation injury. Bakuchiol was shown to be effective to protect mitochondrial functions against oxidative stress.
The ameliorating effects of the cognitive-enhancing Chinese herbs on scopolamine-induced amnesia in rats.:Phytother Res. 2000 Aug;14(5):375-7.Hsieh MT, Peng WH, Wu CR, Wang WH.Institute of Chinese Pharmaceutical Sciences, China Medical College, Taiwan, R.O.C. rei106@tcts1.seed.net.tw
Ameliorating effects were investigated of the cognitive-enhancing Chinese herbs administered orally for 1 week-Panax ginseng (PG), Panax notoginseng (PNG), Dioscorea opposita (DO), Gastrodia elata (GE), Salvia miltiorrhiza (SM), Acorus gramineus (AG), Coptis chinensis (CC), Polygonum multiflorum (PM), Cyperus rotundus (CR) and Psoralea corylifolia (PC)-on the scopolamine (SCOP)-induced amnesia by using a passive avoidance task in rats. Of ten Chinese herbs, only PG, PNG, GE and CC prolonged the SCOP-shortened STL. These results revealed that PG, PNG GE and CC administered orally for 1 week improved the SCOP-induced learning and memory deficit in rats.
Immunomodulatory and antitumour properties of Psoralea corylifolia seeds.:Fitoterapia. 2000 Jun;71(3):223-31.Latha PG, Evans DA, Panikkar KR, Jayavardhanan KK.Ethnomedicine and Ethnopharmacology Division, Tropical Botanic Garden and Research Institute (TBGRI), Palode, Trivandrum, India. telenet@techpark.net
Psoralea corylifolia seed extract has been found to stimulate the immune system in mice. Administration of the extract was found to inhibit EAC ascitic tumour growth and stimulate natural killer cell activity, antibody-dependent cellular cytotoxicity, antibody-forming cells and the antibody complement-mediated cytotoxicity during tumour development.
Inhibition of chemical carcinogenesis by Psoralea corylifolia seeds.:J Ethnopharmacol. 1999 Dec 15;68(1-3):295-8.Latha PG, Panikkar KR.Ethnomedicine and Ethnopharmacology Division, Tropical Botanic Garden and Research Institute, Kerala, India.
Psoralea corylifolia L. (Fabaceae) seeds are extensively used in Ayurvedic medicine. Topical application of 100 mg/kg body weight of the active fraction (AF) of P. corylifolia seeds inhibited the growth and delayed the onset of papilloma formation in mice, initiated with 7,12-dimethyl benz(a) anthracene and promoted using croton oil. The AF at the same dose, when administered orally, inhibited the growth of subcutaneously injected 20-methylcholanthrene (MCA)--induced soft tissue fibrosarcomas significantly. The AF has been shown by gas chromatography analysis to be composed of a mixture of glycerides of fatty acids.
Ancient-modern concordance in Ayurvedic plants: some examples.:Environ Health Perspect. 1999 Oct;107(10):783-9.Dev S.University of Delhi, B.R.A. Centre for Biomedical Research, Delhi, India. ssda@ndf.vsnl.net.in
Ayurveda is the ancient (before 2500 b.c.) Indian system of health care and longevity. It involves a holistic view of man, his health, and illness. Ayurvedic treatment of a disease consists of salubrious use of drugs, diets, and certain practices. Medicinal preparations are invariably complex mixtures, based mostly on plant products. Around 1,250 plants are currently used in various Ayurvedic preparations. Many Indian medicinal plants have come under scientific scrutiny since the middle of the nineteenth century, although in a sporadic fashion. The first significant contribution from Ayurvedic materia medica came with the isolation of the hypertensive alkaloid from the sarpagandha plant (Rouwolfia serpentina), valued in Ayurveda for the treatment of hypertension, insomnia, and insanity. This was the first important ancient-modern concordance in Ayurvedic plants. With the gradual coming of age of chemistry and biology, disciplines central to the study of biologic activities of natural products, many Ayurvedic plants have been reinvestigated. Our work on Commiphora wightti gum-resin, valued in Ayurveda for correcting lipid disorders, has been described in some detail; based on these investigations, a modern antihyperlipoproteinemic drug is on the market in India and some other countries. There has also been concordance for a few other Ayurvedic crude drugs such as Asparagus racemosus, Cedrus deodara, and Psoralea corylifolia.
Micellar electrokinetic capillary chromatography of psoralen and isopsoralen.:Electrophoresis. 1999 Jul;20(9):1895-9.Wang D, Yang G, Engelhardt H, Zhang H.Department of Chemistry, Hebei University, Baoding, China.
In this paper, a micellar electrokinetic capillary chromatography (MEKC) method to analyze the active components of psoralen and isopsoralen in the Chinese herb Psoralea corylifolia L. was developed. It was found that the optimum separation condition for the analysis of these compounds was a buffer solution which contained phosphate, borate and sodium dodecyl sulfate (pH 9.2). Through optimization of the experimental condition, the two isomers could be baseline-separated and their contents in the methanol-extracted sample were quantitatively analyzed.
DNA polymerase and topoisomerase II inhibitors from Psoralea corylifolia.:J Nat Prod. 1998 Mar;61(3):362-6. Erratum in: J Nat Prod. 2003 May;66(5):734. Sun NJ, Woo SH, Cassady JM, Snapka RM.College of Pharmacy, Department of Radiology, Ohio State University, Columbus 43210, USA.
An ethanol extract of Psoralea corylifolia caused strong DNA polymerase inhibition in a whole cell bioassay specific for inhibitors of DNA replication enzymes. Bioassay-directed purification of the active compounds led to the isolation of the new compound corylifolin (1) and the known compound bakuchiol (2) as DNA polymerase inhibitors. On the basis of the structures of 1 and 2, resveratrol (3) was tested and found to be active as a DNA polymerase inhibitor in this bioassay. Neobavaisoflavone (4) was isolated as a DNA polymerase inhibitor, daidzein (5) as a DNA polymerase and topoisomerase II inhibitor, and bakuchicin (6) as a topoisomerase II inhibitor.
The cytotoxicity of psoralidin from Psoralea corylifolia.:Planta Med. 1996 Aug;62(4):353-4.Yang YM, Hyun JW, Sung MS, Chung HS, Kim BK, Paik WH, Kang SS, Park JG.
A cytotoxic coumestan derivative, psoralidin (1), was isolated from the seed of Psoralea corylifolia. The IC50 values of 1 against SNU-1 and SNU-16 carcinoma cell lines were 53 and 203 micrograms/ml, respectively, indicating cytotoxic activity against stomach carcinoma cell lines.
Antiplatelet flavonoids from seeds of Psoralea corylifolia.:J Nat Prod. 1996 Jul;59(7):671-2.Tsai WJ, Hsin WC, Chen CC.National Research Institute of Chinese Medicine, Taipei, Taiwan, Republic of China.
The MeOH extract of the seeds of Psoralea corylifolia L. was found to inhibit the aggregation of rabbit platelets induced by arachidonic acid, collagen, and platelet activating factor. Bioassay-directed fractionation led to the isolation of three flavonoids, isobavachalcone (1), neobavaiso-flavone (2), and bavachin (3). Compounds 1 and 2 inhibited platelet aggregation.
Effect of crude fractions of psoralea corylifolia seed extract on bone calcification.:Planta Med. 1996 Apr;62(2):150-3.
Non-polar crude fractions (Ho-0 and Ho-1) of an acetone extract of Psoralea corylifolia seeds were administrated orally to untreated and experimental rachitic rats. In the biological screening of the fractions, Ho-1, an elution with n-hexane-ethyl acetate by column chromatography over silica gel of the acetone extract, untreated rats showed a significant elevation of the serum inorganic phosphorus and revealed histomorphometrically a significant increase in bone calcification. When Ho-0, an elution with n-hexane, and Ho-1 were administrated to the rachitic rats fed with a vitamin D-free, low-phosphorus diet, they not only increased significantly the concentration of inorganic phosphorus in serum, but also significantly promoted bone calcification. Administration of 30 mg/kg of Ho-1 resulted in a marked decrease of osteoid volume and improvement of hyperosteoidosis in rachitic rats. These results suggested that Ho-0 and Ho-1 are useful as a remedy for bone fracture, osteomalacia, osteoporosis, and related conditions.
Determination of bakuchiol in the fruit of Psoralea corylifolia L.:Zhongguo Zhong Yao Za Zhi. 1995 Nov;20(11):681-3, 704. Chinese.Yao S, Yang B, Xu Z.Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing.
A GC method for the determination of bakuchiol of described. GC conditions for analysing are as follows: stationary phase: SE-30: injector temperature: 250 degrees C: column temperature: 200 degrees C and detector: FID. The linear range of this method is 0.24-2.4 micrograms and the average recovery is 98.82%, with a relative standard deviation of 1.58%. This method has been successfully used to determine the content of bakuchiol in the samples on the market in 11 different areas, the contents being 1%-7%.
Review of constituents in fruits of Psoralea corylifolia L.:Zhongguo Zhong Yao Za Zhi. 1995 Feb;20(2):120-2, 128. Review. Chinese.Ji L, Xu Z.Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing.
Thirty-five constituents in the fruits of Psoralea corylifolia (identified since 1933) and their main physiological activities and clinical uses have been reviewed.
Application of the regression orthogonal design to the fertilization of Psoralea corylifolia L. for optimum yield.:Zhongguo Zhong Yao Za Zhi. 1992 Aug;17(8):466-8, 510. Chinese.Ding D, Lu J, Chen X, Xu D, Huang W, Liu S.Institute of Medicinal Plant Cultivation, Sichuan Academy of Traditional Chinese Medicine, Nanchuan
This paper probes into the determination of the optimum amount of fertilization by applying regression orthogonal design. After a significance test by regression equation, a regression equation can be set up, i. e. Y = 25.21 + 9.18 Xn + 15.68 Xp - 0.4 Xn2 - 0.98 Xp2 + 0.062 Xn Xp. This equation can be used to describe the relationship between yield and amount of fertilization.
Nutritive physiology of Psoralea corylifolia L.:Zhongguo Zhong Yao Za Zhi. 1990 Sep;15(9):524-6, 575. Chinese.Liu S, Ding D, Lu J, Xu D, Chen X, Huang W.Institute of Medicinal Plant Cultivation, Sichuan Academy of Traditional Chinese Medicine, Nanchuan.
Our study has shown that P. corylifolia absorbs and accumulates N2 and K2O most speedily in the periods of branching, flowering and fruiting, while P2O5 in the period of full flowering and fruiting. P2O5 is accumulated mainly in the seed and K2O in the stem. The nutriment accumulation is positively correlated with that of dry substances. To produce 100 kg of P. corylifolia an absorption of 10.59 kg of N2, 3.68 kg of P2O5 and 10.21 kg of K2O is needed.
Effect of seed soaking in plant hormone and trace element fertilizer on the growth of Psoralea corylifolia L.:Zhongguo Zhong Yao Za Zhi. 1990 Aug;15(8):466-7, 511. Chinese.Ding D, Lu J, Huang WX, Xu D, Liu S, Chen X.Institute of Medicinal Plant Cultivation, Sichuan Academy of Traditional Chinese Medicine, Nanchuan.
Our study shows that seed soaking in triacontanol, ammonium molybdate and gibberellin can promote the growth of P. corylifolia, reduce its premature flower and fruit drop, raise the fruit-bearing rate by 6.5-17.4%, and fruit-bearing number by 21.6-28.4%, so as to increase the yield per unit area by 31.5-34.8%. Seed soaking in naphthylacetic acid and boric acid does not give such a marked effect.
Biological characteristics of Psoralea corylifolia L.:Zhongguo Zhong Yao Za Zhi. 1990 Apr;15(4):206-9, 253-4. Chinese.Zhang X, Zhou C, Lu J, Ding D, Xu D, Huang W, Liu S, Chen X.Institute of Medical Plant Cultivation, Sichuan Academy of Traditional Chinese Medicine and Pharmacy, Nanchuan.
A study has been made on the biological characteristics of Psoralea corylifolia under general cultivating conditions. To search for the laws of growth and development so as to facilitate further studies on the cultivating techniques for higher production of Psoralea corylifolia.
Fate of leucine in the biosynthesis of bakuchiol, a meroterpene from Psoralea corylifolia.:Indian J Biochem Biophys. 1989 Dec;26(6):394-6.Banerji A, Chintalwar GJ.
Significant incorporations of labelled leucine, valine and isovaleric acid into the meroterpene, bakuchiol (1) isolated from the medicinal plant, Psoralea corylifolia have been observed. Degradation experiments show that labels from these substrates find their way into both phenylpropane derived as well as terpenic part of 1 thereby indicating that none of the known pathways is operative in the case of 1. It is suggested that these substrates are metabolised to CO2 which is then incorporated into 1.
Cytotoxicity of corylifoliae fructus. II. Cytotoxicity of bakuchiol and the analogues.:
Bakuchiol is a major component of Corylifoliae Fructus (Psoralea corylifolia L.) and has been clarified to have cytotoxic activity. The chemical structure-cytotoxic activity relationship of bakuchiol was investigated by means of cytotoxic activity of synthesized analogues of bakuchiol and phenol. It was proved that an alkyl group was necessary for cytotoxic activity. But the double bonds in the unsaturated hydro-carbon group exerted but little influence on the cytotoxic activity. The cytotoxic activity of bakuchiol was the strongest as compared with that of the analogues examined.
Cytotoxicity of Corylifoliae fructus. I. Isolation of the effective compound and the cytotoxicity.:
The ethanol extract of Psoraleae Fructus (Psoralea corylifolia L.) was found to have cytotoxic activity against L929-cells in cell culture. The active compound was isolated by column chromatography on silica gel and identified as bakuchiol by means of spectral evidence. The cytotoxic activity of bakuchiol in cell culture was observed in short time and found to be unreversible. The mechanism of the cytotoxic activity was considered to be due to an injury of cell membrane from electron microscopic observation and hemolytic activity.
Measurement of MEFV in 66 cases of asthma in the convalescent stage and after treatment with Chinese herbs.:Zhong Xi Yi Jie He Za Zhi. 1989 Nov;9(11):658-9, 644. Chinese.Fu JX.
This paper reported the measurement of maximal expiratory flow-volume curve (MEFV) for 66 cases of asthmatics in the convalescent stage. Among which the data of FEV, PEF, V75, V50, V25 in 35 cases (53.03% of the total) gave different abnormal as compared with healthy persons. It showed that in the convalescent stage, most of the asthmatics still possessed obstruction of airways and chiefly of small airways. 35 cases of asthmatics in the convalescent stage was given the Chinese herbal decoction of chiefly invigorating Kidney (Viscum coloratum 15g, Psoralea corylifolia 15g, Eucommia ulmoides 15g, Lycium chinense 9g, Tussilago farfara 15g, Artemisia capillaris 9g, and Pogostemon cablin 9g as daily dosage) for treatment of 10 weeks and measuring MEFV curves to observe their changes before and after treatment. The results showed that different parameters of MEFV was improved in some extent which suggested that the airway obstruction of asthmatics in the convalescent stage was reversible. In discussion, the authors indicated that the prompt treatment for asthmatics in the convalescent stage was conductive early to prevent emphysema and confirmed that the treatment with Chinese herbs of chiefly invigorating Kidney deserved to be propagated.
Plant antimutagenic agents, 3. Coumarins.:J Nat Prod. 1988 Nov-Dec;51(6):1148-52.Wall ME, Wani MC, Manikumar G, Hughes TJ, Taylor H, McGivney R, Warner J.Research Triangle Institute, Research Triangle Park, North Carolina 22709.
Several coumarins were isolated from crude plant extracts by means of an antimutagenic assay procedure. These coumarins included psoralen from Psoralea corylifolia and imperatorin and osthol from Selinum monniere. Studies of structure-activity relationships of these and several other available coumarins were carried out with four mutagens. All of the coumarins were nontoxic and in particular showed high activity in the inhibition of the mutagenicity of benzo[a]pyrene.
Plant antimutagenic agents, 2. Flavonoids.:J Nat Prod. 1988 Nov-Dec;51(6):1084-91.Wall ME, Wani MC, Manikumar G, Abraham P, Taylor H, Hughes TJ, Warner J, McGivney R.Research Triangle Institute, Research Triangle Park, North Carolina 22709.
A number of known prenylated flavonoids were isolated from Psoralea corylifolia using an assay procedure based on inhibition of the mutagenic action of 2-aminoanthracene on Salmonella typhimurium (T-98). All of these compounds were toxic rather than antimutagenic or desmutagenic. Bakuchiol [17], a known prenylated phenolic terpene, was also isolated; its activity was not due to toxicity. Biochanin A [4], a known isoflavone, was similarly isolated from Cicer arientinum and was active and nontoxic. Some of the above flavonoids were studied for inhibition of the mutagenicity of several different mutagens with results depending upon the structure of the flavonoid and the mutagen.
Kinetics of the anti-staphylococcal activity of bakuchiol in vitro (author's transl).:Arzneimittelforschung. 1976 Apr;26(4):486-9. German.Kaul R.
The growth curve of Staphylococcus aureus in a liquid medium with and without bakuchiol, the main component of Psoralea corylifolia Linn., has been studied for in vitro evaluation of the antibacterial activity. The dynamics of the antibacterial action has also been studied by finding the generation time and number of generations of the microbial growth before stationary phase is reached. This study of the growth curve of the test bacteria gives in general an accurate method of evaluating the in vitro antibacterial activity of drugs with exact mathematical implications, besides serving also as a means for studying the kinetics as well as the mode of antibacterial action.
Claims: Information this web site presented is meant for Nutritional Benefit and as an educational starting point only, for use in maintenance and promotion good health in cooperation with a common knowledge base reference...Furthermore,it based solely on the traditional and historic use or legend of a given herb from the garden of Adonis. Although every effort has been made to ensure its accurate, please note that some info may be outdated by more recent scientific developments......
Pharmakon Warning: The order of knowledge is not the transparent order of forms and ideas,as one might be tempted retrospectively to interpret it; it is the antidote....(Dissemination,Plato's Pharmacy,II.The Ingredients:Phantasms,Festivals,and Paints;138cf. Jacques Derrida.).
And as it happens,the technique of imitation,along with the production of the simulacrum,has always been in Plato's eyes manifestly magical,thaumaturgical:......and the same things appear bent and straight to those who view them in water and out,or concave and convex,owing to similar errors of vision about colors, and there is obviously every confusion of this sort in our souls.And so scene painting (skiagraphia) in its exploitation of this weakness of four nature falls nothing short of witchcraft (thaumatopoia), and so do jugglery and many other such contrivances.(Republic X,602c-d;cf.also 607c).
seminal trace...Psoralea fruit Extract.Babchi seed extract.Psoralea corylifolia seed extract,Psoralea extract.10:1.Psoralen,C17H18O6,CAS No:10523-56-5; isopsoralen,C11H6O3,CAS No:523-50-2...
Phytochemical info of Psoralea fruit Extract.
Study on micro-wave processed of Psoralea corylifolia with uniform and regression analysis.:Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1167-70. Chinese.Guo YH, Jia TZ, Lin GM.Liaoning Traditional Chinese Medicine University, Shenyang 110032, China.
Scientific References:
Claims & Warning:
