Aralia elata Root Bark and Its Extracts:Applications and Study!
Contents
Microscopy Aralia Root.:
On root cross cut is seen a layer of heavily peeling cork. Bark consists of parenchyma cells with thin walls, among which are located secretory ducts from 7 to 20 in diameter in the way of concentric belts. Parenchyma cells located around secretory ducts and medullary rays are filled with starch grains. Starch grains are simple and binate to octosyllabic. In the outer part of cork can be seen druses of calcium oxalate. Cork is separated from wood by a narrow layer of cambium. Wood is annular-vessel. Medullary rays are monostichous to pentastichous. After maceration, in the specimen are seen spiral and porous vessels with simple or marginate pores, fibrous tracheids and libriform fibers.
Qualitative Reactions:
A stock analytical sample is pulverized down to the particle size passing through a 7mm sieve. About 1 grams of mill stock is put into a 50ml flask, then add 20ml of methyl alcohol and boil on water bath (bath temperature: 80-85) with a reflux condenser for 1 hour; 0.02ml of 5min-settled extract is applied with the help of a micropipette on the starting line of chromatographic 20 by 20cm plate with a fixed layer of coarse, macroporous silica gel. For a reference spot is applied 0.01ml of 0.6% solution of saparal* in methyl alcohol (50 g). In 10 minutes, the plate is put into a chamber with a mixture of solvents: chloroform-methyl alcohol-water (61:32:7) and chromatograph by an ascending method. When solvent front reaches the plate end, it is taken out of the chamber, dried in air for 10 minutes, sprinkled with a 20% solution of sulfuric acid and heat in a drying cabinet at 105 for 10 minutes.
On the plate must appear spots of cherry color on the level of aralozide spots in saparal. It is allowed to have additional spots both of cherry and other colors.
Reference:
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- 1.Aralia elata Root Bark and Its Extracts:Applications and Study!
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